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Catalog Number Size Price
LS-B5455-50 50 µg (1 mg/ml) $440 
SMAD3 Antibody - Anti-SMAD3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
SMAD3 Antibody - SMAD3 monoclonal antibody clone 2C12. Western blot of SMAD3 expression in U-2 OS.
SMAD3 Antibody - SMAD3 monoclonal antibody clone 2C12. Western blot of SMAD3 expression in human colon.
SMAD3 Antibody - Detection limit for recombinant GST tagged SMAD3 is approximately 0.03 ng/ml as a capture antibody.
SMAD3 Antibody - Anti-SMAD3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
SMAD3 Antibody - SMAD3 monoclonal antibody clone 2C12. Western blot of SMAD3 expression in U-2 OS.
SMAD3 Antibody - SMAD3 monoclonal antibody clone 2C12. Western blot of SMAD3 expression in human colon.
SMAD3 Antibody - Detection limit for recombinant GST tagged SMAD3 is approximately 0.03 ng/ml as a capture antibody.
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IHC‑plus™ Monoclonal Mouse anti‑Human SMAD3 Antibody (clone 2C12, IHC, WB) LS‑B5455

IHC‑plus™ Monoclonal Mouse anti‑Human SMAD3 Antibody (clone 2C12, IHC, WB) LS‑B5455

Note: This antibody replaces LS-C133305
Antibody:
SMAD3 Mouse anti-Human Monoclonal (2C12) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$440
LS-B5455-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
SMAD3 Mouse anti-Human Monoclonal (2C12) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
SMAD3 antibody LS-B5455 is an unconjugated mouse monoclonal antibody to human SMAD3. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human SMAD3
Synonyms
SMAD3 | HSPC193 | HMAD-3 | HsT17436 | LDS1C | LDS3 | Mad homolog JV15-2 | MAD3 | MADH3 | Mothers against DPP homolog 3 | JV15-2 | SMA- and MAD-related protein 3 | SMAD family member 3 | MAD homolog 3 | HSMAD3 | Mad protein homolog | SMAD 3
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
2C12
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Immunogen
SMAD3 (NP_005893, 120 aa ~ 222 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human SMAD3
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using tissue lysates, cell line lysates and the recombinant protein used as the immunogen.
Presentation
PBS, pH 7.4
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SMAD3
P84022 NM_005902 NP_005893.1

Validation

SMAD3 Antibody - Anti-SMAD3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-SMAD3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-SMAD3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ SMAD3 Antibody (clone 2C12) for IHC, WB/Western, ELISA LS-B5455 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/22/2024