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Catalog Number	Size	Price
LS-C368536-30	30 µl (1 mg/ml)	$263
LS-C368536-100	100 µl (1 mg/ml)	$315
LS-C368536-200	200 µl (1 mg/ml)	$379

SMAD3 Antibody - Immunohistochemical analysis of SMAD3 (pS213) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

SMAD3 Antibody - Immunofluorescent analysis of SMAD3 (pS213) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

SMAD3 Antibody - Western blot analysis of SMAD3 (pS213) expression in A549 (A); Jurkat (B) whole cell lysates.

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Polyclonal Rabbit anti‑Human SMAD3 Antibody (phospho‑Ser213, IHC, IF, WB) LS‑C368536

Name: Anti-SMAD3 Antibody | Rabbit anti-Human Polyclonal ICC,IF,IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C368536-30
Price: 263.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

SMAD3 Rabbit anti-Human Polyclonal (pSer213) Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human, Mouse, Rat, Pig, Chicken

Format:

Unconjugated, Unmodified

$263

LS-C368536-30

$315

LS-C368536-100

$379

LS-C368536-200

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

SMAD3 Rabbit anti-Human Polyclonal (pSer213) Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human, Mouse, Rat, Pig, Chicken

Format:

Unconjugated, Unmodified

Specifications

Description

SMAD3 antibody LS-C368536 is an unconjugated rabbit polyclonal antibody to SMAD3 (pSer213) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB.

Target

Human SMAD3

Synonyms

See All SMAD3 Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat, Pig, Chicken (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunoaffinity chromatography

Modifications

Unmodified

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the central region of human SMAD3.

Epitope

pSer213

Specificity

Recognizes endogenous levels of SMAD3 (pS213) protein.

Applications

IHC
IHC - Paraffin (1:100 - 1:200)
ICC (1:100 - 1:500)
Immunofluorescence
Western blot (1:500 - 1:1000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol

Storage

Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About SMAD3

Uniprot: P84022 NCBI: NM_005902 NP_005893.1