Human IL-1B / IL-1 Beta

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

CRM56

Unconjugated

Affinity purified

Unmodified

Human IL1B

• Flow Cytometry
• ELISA
• Neutralization

The CRM56 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human interleukin-1beta (IL-1beta) in combination with the biotin CRM57 (LS-C106945) antibody for detection and recombinant human IL-1beta as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1 - 4 ug/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/ml - 8 pg/ml should be included in each ELISA plate. The Functional Grade Purified CRM56 antibody has been tested for neutralization of IL-1beta bioactivity. The fluorochrome-conjugated CRM56 antibody has been tested by intracellular staining for flow cytometric analysis. The fluorochrome- conjugated CRM56 antibody is offered in 2 formats: - ug size: has been tested by intracellular staining with flow cytometric analysis and can be used at less than or equal to 0.50 ug/million cells/100 ul staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by intracellular staining and flow cytometric analysis of activated human PBMC and can be used at 20 ul per million cells 100 ul blood.

PBS, pH 7.2, 150 mM NaCl, 0.09% Sodium Azide

Store at 4°C, avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P01584 NCBI: NM_000576 NP_000567.1