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Antibodies > IL17A > LS-C805982
 
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Catalog Number Size Price
LS-C805982-100 100 µl (1 mg/ml) $379 
LS-C805982-200 200 µl (1 mg/ml) $421 
IL17A Antibody - 1:100 staining human Melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
IL17A Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
IL17A Antibody - Western blot analysis of extracts of HeLa cells using IL17A antibody. The lane on the left is treated with the antigen-specific peptide.
IL17A Antibody - 1:100 staining human Melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
IL17A Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
IL17A Antibody - Western blot analysis of extracts of HeLa cells using IL17A antibody. The lane on the left is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human IL17A Antibody (IHC, IF, WB) LS‑C805982

Polyclonal Rabbit anti‑Human IL17A Antibody (IHC, IF, WB) LS‑C805982

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Antibody:
IL17A Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C805982-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
IL17A Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
IL17A antibody LS-C805982 is an unconjugated rabbit polyclonal antibody to IL17A from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human IL17A
Synonyms
IL17A | CTLA-8 | CTLA8 | Interleukin-17A | Interleukin 17A | IL-17 | IL-17A | IL17
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human IL17A.
Specificity
IL17A Antibody detects endogenous levels of total IL17A.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:20 - 1:50)
  • Western blot (1:200 - 1:1500)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL17A
Q16552 NM_002190 NP_002181.1

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IL17A Antibody - Untreated (A) and PMA/ionomycin -stimulated (B) human PBMCs were stained using PE-conjugated mouse anti-human CD3 antibody and APC-conjugated antibody Interleukin 17 (IL-17, CTLA8, IL-17A, Interleukin-17A precursor, Cytotoxic T-lymphocyte-associated antigen 8) (APC) This image was taken for the unconjugated form of this product. Other forms have not been tested.
IL17A Antibody (APC) LS‑C37030
Species: Human
Applications: Flow Cytometry
IL17A Antibody - Biotin Rabbit Anti-Rat IL-17A Antibody - Western Blot. Western Blot showing detection of Rat IL-17A. 50 ng of Rat IL-17A (Lane 1) was run on a 4-20% gel and transferred to 0.45 micron nitrocellulose. After blocking with 5% Blotto (B501-0500) 30 min at 20?, Anti-Rat IL-17A (RABBIT) Antibody Biotin Conjugated Anti-Rat IL-17A (RABBIT) Antibody Biotin Conjugated - 212-406-B32 secondary antibody was used at 1:5000 in Blocking Buffer for Fluorescent Western Blot (p/n MB-070). HRP Streptavidin (p/n S000-03) was used at 1:40000 in MB-070 for 30 min at 20? and imaged using the Bio-Rad VersaDoc 4000 MP. Arrow indicates correct 15 kD molecular weight position expected for Rat IL-17A. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IL17A Antibody (Biotin) LS‑C154151
Species: Rat, Mouse
Applications: IHC, Western blot, ELISA
IL17A Antibody - Western Blot (reducing) of IL-17 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IL17A Antibody LS‑C104427
Species: Human
Applications: Western blot, ELISA
Product
IL17A Antibody (clone 41802, PE) LS‑C37031
Species: Human
Applications: Flow Cytometry
See all IL17A Antibodies

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/24/2024
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