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Catalog Number Size Price
LS-C358786-30 30 µl (1 mg/ml) $263 
LS-C358786-100 100 µl (1 mg/ml) $315 
LS-C358786-200 200 µl (1 mg/ml) $379 
IRS1 Antibody - Immunohistochemical analysis of IRS1 (pS307) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
IRS1 Antibody - Immunofluorescent analysis of IRS1 (pS307) staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
IRS1 Antibody - Western blot analysis of IRS1 (pS307) expression in HEK293T (A); NS-1 (B) whole cell lysates.
IRS1 Antibody - Immunohistochemical analysis of IRS1 (pS307) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
IRS1 Antibody - Immunofluorescent analysis of IRS1 (pS307) staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
IRS1 Antibody - Western blot analysis of IRS1 (pS307) expression in HEK293T (A); NS-1 (B) whole cell lysates.
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Polyclonal Rabbit anti‑Human IRS1 Antibody (phospho‑Ser307, IHC, IF, WB) LS‑C358786

Polyclonal Rabbit anti‑Human IRS1 Antibody (phospho‑Ser307, IHC, IF, WB) LS‑C358786

Antibody:
IRS1 Rabbit anti-Human Polyclonal (pSer307) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Monkey, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$263
LS-C358786-30
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
IRS1 Rabbit anti-Human Polyclonal (pSer307) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Monkey, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
IRS1 antibody LS-C358786 is an unconjugated rabbit polyclonal antibody to IRS1 (pSer307) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB.
Target
Human IRS1
Synonyms
IRS1 | IRS-1 | HIRS-1 | Insulin receptor substrate 1
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human IRS1 (pS307).
Epitope
pSer307
Specificity
Recognizes endogenous levels of IRS1 (pS307) protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IRS1
P35568 NM_005544 NP_005535.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/21/2024