Mouse Leptin

Rabbit

Mouse (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated. Also available conjugated with Biotin.

Immunoaffinity purified

Unmodified

E.coli derived recombinant Murine Leptin.

Mouse Leptin

• Western blot (0.1 - 0.2 µg/ml)
• ELISA (0.5 - 2 µg/ml)

Immunohistochemistry: This antibody stained 4% PFA, paraffin-embedded sections of Trichuris muris infected murine tissue. The primary antibody was incubated at 0.20 ug/mL overnight at 4°C and the secondary antibody was a cyanine-3 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100mM citric acid was used. Information and photo are courtesy of David Artis, University of Pennsylvania. Optimal concentrations and conditions may vary. ELISA: To detect mLeptin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine Leptin (LS-C104759) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mLeptin. Western Blot: To detect mLeptin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mLeptin is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Lyophilized from PBS, pH 7.2

500 ul sterile ddH20

Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P41159 NCBI: NM_000230 NP_000221.1