Human MIF

Chicken

Human, Monkey (tested or 100% immunogen sequence identity)

IgY Polyclonal

Unconjugated

Purified

Unmodified

Eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to aa 2-32 of Human MIF conjugated to keyhole limpet hemocyanin (KLH). Peptide sequence: PMFIVNTNVPRASVPDGF- LSELTQQLAQATG. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Mouse, Rat, Pig (94%); Sheep, Panda, Bovine, Horse, Rabbit (89%); Hamster (83%).

aa2-32

Recognizes human mature Migration Inhibitory Factor, Macrophage (MIF) at 12.3kD. The MIF gene encodes a protein of 115aa. The initiating methionine is cleaved leaving a mature protein of 114aa.

• Western blot (1:500 - 1:1000)
• ELISA (1:1000 - 1:2000)

Suitable for use in ELISA and Western Blot. Western Blot: 1:500-1:1000. Detects human MIF from supernatants or lysates of 2x1E6 endotoxin-stimulated PBMC stimulated for 24 hours with 1% human serum plus 10 mg/ml E. coli LPS. ELISA: 1:1000-1:2000. Assayed against goat anti-chicken IgG (H&L) in a standard capture ELISA using ABTS as a substrate for 30 min at RT.

Lyophilized from PBS, pH 7.2, 0.01% Sodium Azide, No stabilizing proteins added

Reconstitute using sterile distilled H2O.

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P14174 NCBI: NM_002415 NP_002406.1