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Catalog Number Size Price
LS-C746899-20 20 µl $275 
LS-C746899-100 100 µl $389 
NFKB1 / NF-Kappa-B Antibody - Immunohistochemistry of paraffin-embedded human gastric cancer using NFKB1 antibody at dilution of 1:100 (40x lens).
NFKB1 / NF-Kappa-B Antibody - Immunohistochemistry of paraffin-embedded human tonsil using NFKB1 antibody at dilution of 1:100 (40x lens).
NFKB1 / NF-Kappa-B Antibody - Immunofluorescence analysis of MCF7 cells using NFKB1 antibody.
NFKB1 / NF-Kappa-B Antibody - Western blot analysis of extracts of various cell lines, using NFKB1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
NFKB1 / NF-Kappa-B Antibody - Immunoprecipitation analysis of 150ug extracts of MCF-7 cells using 3ug NFKB1 antibody. Western blot was performed from the immunoprecipitate using NFKB1 antibody at a dilition of 1:500.
NFKB1 / NF-Kappa-B Antibody - Immunohistochemistry of paraffin-embedded human gastric cancer using NFKB1 antibody at dilution of 1:100 (40x lens).
NFKB1 / NF-Kappa-B Antibody - Immunohistochemistry of paraffin-embedded human tonsil using NFKB1 antibody at dilution of 1:100 (40x lens).
NFKB1 / NF-Kappa-B Antibody - Immunofluorescence analysis of MCF7 cells using NFKB1 antibody.
NFKB1 / NF-Kappa-B Antibody - Western blot analysis of extracts of various cell lines, using NFKB1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
NFKB1 / NF-Kappa-B Antibody - Immunoprecipitation analysis of 150ug extracts of MCF-7 cells using 3ug NFKB1 antibody. Western blot was performed from the immunoprecipitate using NFKB1 antibody at a dilition of 1:500.
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Polyclonal Rabbit anti‑Human NFKB1 / NF‑Kappa‑B Antibody (IHC, IF, WB) LS‑C746899

Polyclonal Rabbit anti‑Human NFKB1 / NF‑Kappa‑B Antibody (IHC, IF, WB) LS‑C746899

Antibody:
NFKB1 / NF-Kappa-B Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C746899-20
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
NFKB1 / NF-Kappa-B Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
NF-Kappa-B antibody LS-C746899 is an unconjugated rabbit polyclonal antibody to human NF-Kappa-B (NFKB1). Validated for IF, IHC, IP and WB.
Target
Human NFKB1 / NF-Kappa-B
Synonyms
NFKB1 | Ebp- 1 | EBP-1 | NF-KappaB p50 | NFkappaB | NFKB-p50 | KBF1 | NF-kappa-B | NF-kappaB | NF-kB1 | NFKB-p105 | p105 | p50 | DNA binding factor KBF1 | DNA-binding factor KBF1 | NF-kappabeta
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 740-964 of human NFKB1 (NP_001158884.1). AAGADPLVENFEPLYDLDDSWENAGEDEGVVPGTTPLDMATSWQVFDILNGKPYEPEFTSDDLLAQGDMKQLAEDVKLQLYKLLEIPDPDKNWATLAQKLGLGILNNAFRLSPAPSKTLMDNYEVSGGTVRELVEALRQMGYTEAIEVIQAASSPVKTTSQAHSLPLSPASTRQQIDELRDSDSVCDSGVETSFRKLSFTESLTSGASLLTLNKMPHDYGQEGPL
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:20 - 1:100)
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:20 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 85kDa/105kDa, while the observed MW by Western blot was 120kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NFKB1 / NF-Kappa-B
P19838 NM_003998 NP_003989.2

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Requested From: United States
Date Requested: 11/24/2024