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Antibodies > NFKB1 / NF-Kappa-B > LS-C801049
 
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Catalog Number Size Price
LS-C801049-100 100 µl (1 mg/ml) $379 
LS-C801049-200 200 µl (1 mg/ml) $421 
NFKB1 / NF-Kappa-B Antibody - 1:100 staining human breast carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
NFKB1 / NF-Kappa-B Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
NFKB1 / NF-Kappa-B Antibody - Staining HeLa cells treated with EGF 200nM 5' cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
NFKB1 / NF-Kappa-B Antibody - Western blot analysis of NF-kappaB p105/p50 phosphorylation expression in LPS treated HeLa whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
NFKB1 / NF-Kappa-B Antibody - Western blot analysis of Phospho-NF-kappaB p105/p50 (Ser927) expression in various lysates
NFKB1 / NF-Kappa-B Antibody - 1:100 staining human breast carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
NFKB1 / NF-Kappa-B Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
NFKB1 / NF-Kappa-B Antibody - Staining HeLa cells treated with EGF 200nM 5' cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
NFKB1 / NF-Kappa-B Antibody - Western blot analysis of NF-kappaB p105/p50 phosphorylation expression in LPS treated HeLa whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
NFKB1 / NF-Kappa-B Antibody - Western blot analysis of Phospho-NF-kappaB p105/p50 (Ser927) expression in various lysates
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Polyclonal Rabbit anti‑Human NFKB1 / NF‑Kappa‑B Antibody (phospho‑Ser927, IHC, IF, WB) LS‑C801049

Polyclonal Rabbit anti‑Human NFKB1 / NF‑Kappa‑B Antibody (phospho‑Ser927, IHC, IF, WB) LS‑C801049

Submit Review LSBio Guarantee
Antibody:
NFKB1 / NF-Kappa-B Rabbit anti-Human Polyclonal (pSer927) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801049-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
NFKB1 / NF-Kappa-B Rabbit anti-Human Polyclonal (pSer927) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
NF-Kappa-B antibody LS-C801049 is an unconjugated rabbit polyclonal antibody to NF-Kappa-B (NFKB1) (pSer927) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human NFKB1 / NF-Kappa-B
Synonyms
NFKB1 | Ebp- 1 | EBP-1 | NF-KappaB p50 | NFkappaB | NFKB-p50 | KBF1 | NF-kappa-B | NF-kappaB | NF-kB1 | NFKB-p105 | p105 | p50 | DNA binding factor KBF1 | DNA-binding factor KBF1 | NF-kappabeta
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human NF- kappaB p105/p50 around the phosphorylation site of Serine 927.
Epitope
pSer927
Specificity
Phospho-NF- kappaB p105/p50 (Ser927) Antibody detects endogenous levels of NF- kappaB p105/p50 only when phosphorylated at Serine 927.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NFKB1 / NF-Kappa-B
P19838 NM_003998 NP_003989.2

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Featured Products

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NFKB1 / NF‑Kappa‑B Antibody (N‑Terminus) LS‑B8072
Species: Human, Chicken
Applications: IHC, IHC - Paraffin, Western blot
NFKB1 / NF-Kappa-B Antibody - NFKB1 antibody Western blot of 1 Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
NFKB1 / NF‑Kappa‑B Antibody (C‑Terminus) LS‑C112312
Species: Human
Applications: Western blot
NFKB1 / NF-Kappa-B Antibody - Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml]
NFKB1 / NF‑Kappa‑B Antibody (clone 3F6) LS‑C133346
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
NFKB1 / NF-Kappa-B Antibody - Anti-NFKB1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.
IHC‑plus™ NFKB1 / NF‑Kappa‑B Antibody (clone 4D1) LS‑B1668
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
NFKB1 / NF-Kappa-B Antibody - Anti-NFKB1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
IHC‑plus™ NFKB1 / NF‑Kappa‑B Antibody (N‑Terminus) LS‑B395
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Product
NFKB1 / NF‑Kappa‑B Antibody (Biotin) LS‑C528248
Species: Human
Applications: Western blot, ELISA
See all NFKB1 / NF-Kappa-B Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/3/2024
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