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Catalog Number Size Price
LS-C357483-10 10 µg $318 
LS-C357483-100 100 µg (0.5 mg/ml) $470 
NOD1 Antibody - IHC analysis of CARD4 using anti-CARD4 antibody. CARD4 was detected in immunocytochemical section of Hela cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CARD4 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
NOD1 Antibody - IHC analysis of CARD4 using anti-CARD4 antibody. CARD4 was detected in immunocytochemical section of SMMC-7721 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CARD4 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
NOD1 Antibody - CARD4 antibody IHC-paraffin: Human Placenta Tissue.
NOD1 Antibody - IHC analysis of CARD4 using anti-CARD4 antibody. CARD4 was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CARD4 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
NOD1 Antibody - CARD4 antibody Western blot. All lanes: Anti CARD4 at 0.5 ug/ml. Lane 1: A549 Whole Cell Lysate at 40 ug. Lane 2: Rat Cardiac Muscle Tissue Lysate at 50 ug. Predicted band size: 107 kD. Observed band size: 107 kD.
NOD1 Antibody - IHC analysis of CARD4 using anti-CARD4 antibody. CARD4 was detected in immunocytochemical section of Hela cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CARD4 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
NOD1 Antibody - IHC analysis of CARD4 using anti-CARD4 antibody. CARD4 was detected in immunocytochemical section of SMMC-7721 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CARD4 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
NOD1 Antibody - CARD4 antibody IHC-paraffin: Human Placenta Tissue.
NOD1 Antibody - IHC analysis of CARD4 using anti-CARD4 antibody. CARD4 was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CARD4 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
NOD1 Antibody - CARD4 antibody Western blot. All lanes: Anti CARD4 at 0.5 ug/ml. Lane 1: A549 Whole Cell Lysate at 40 ug. Lane 2: Rat Cardiac Muscle Tissue Lysate at 50 ug. Predicted band size: 107 kD. Observed band size: 107 kD.
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Polyclonal Rabbit anti‑Human NOD1 Antibody (aa1‑160, IHC, WB) LS‑C357483

Polyclonal Rabbit anti‑Human NOD1 Antibody (aa1‑160, IHC, WB) LS‑C357483

Note: This antibody replaces LS-C388131
Antibody:
NOD1 Rabbit anti-Human Polyclonal (aa1-160) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C357483-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
NOD1 Rabbit anti-Human Polyclonal (aa1-160) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
NOD1 antibody LS-C357483 is an unconjugated rabbit polyclonal antibody to NOD1 (aa1-160) from human. It is reactive with human and rat. Validated for IHC and WB.
Target
Human NOD1
Synonyms
NOD1 | CARD4 | CLR7.1 | NLRC1
Host
Rabbit
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human CARD4 recombinant protein (Position: M1-M160). Human CARD4 shares 82% amino acid (aa) sequence identity with mouse CARD4.
Epitope
aa1-160
Specificity
Highly expressed in adult heart, skeletal muscle, pancreas, spleen and ovary. Also detected in placenta, lung, liver, kidney, thymus, testis, small intestine and colon.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NOD1
Q9Y239 NM_006092 NP_006083.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 10/31/2024