Human NOS1 / nNOS

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

Ved in human, rat and mouse. IMMUNOGEN: Synthetic peptide corresponding to amino acids 724-739 of human brain NOS, coupled to KLH.

aa724-739

Reactive with human brain NOS-I (nNOS). Antibody binding is neutralized in Western blot by pre-adsorption with the immunogen peptide. This sequence is highly conserved in human, rat and mouse.

• IHC
• Immunofluorescence
• Western blot (1 - 10 µg/ml)
• ELISA

Immunohistochemistry: Use at 5-10 ug/ml on rat neuronal sections fixed with 3.5% paraformaldehyde. Incubation overnight at 4?C with a 1:100-1:500 dilution. ABC (peroxidase) detection. Positive staining of Purkinje cells and Layer 5 primary neurons. Western blot: 1-10 ug/ml with chemiluminescent detection. Higher concentrations of the antibody may be necessary if colorimetric detection is used. Antibody dilution will also vary with the concentration of antigen. Then antibody should recognize a 155kD protein. ELISA: Use at 1.0 ug/ml or lower on plates coated with 50-100 ng of peptide antigen.

PBS, pH 7.4, 0.1% Sodium Azide, 0.1% BSA

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P29475 NCBI: NM_000620 NP_000611.1