Human PDCD4

Rabbit

Human, Mouse, Rat, Xenopus (tested or 100% immunogen sequence identity)

Mouse, Rat, Xenopus (at least 90% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding amino acids near the carboxyl terminus of human Pdcd4 protein.

A BLAST analysis was used to suggest cross-reactivity with Pdcd4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with Pdcd4 from other sources has not been determined. The antibody reacts with Pdcd4 protein that is either phosphorylated or non-phosphorylated at Ser457.

• IHC
• IHC - Paraffin (10 µg/ml)
• Western blot (1:1000 - 1:10000)
• ELISA (1:10000 - 1:100000)

Immunohistochemistry: LS-B65 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B65 was determined to be 10 ug/ml.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q53EL6 NCBI: NM_014456 NP_055271.2