Rat PECAM-1 / CD31

Mouse

Rat (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

TLD-3A12

Unconjugated

Protein G purified

Low Endotoxin. Also available Unmodified.

Activated, Lewis rat derived microglial cells

Recognizes rat PECAM-1 (CD31), a 661 amino acid type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes. Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen. This clone has been reported as suitable for use in IHC on formalin-fixed paraffin-embedded sections pre-treated with 0.2 M boric acid, pH7.0. Has been shown to be cross-reactive with endothelial cells derived from rhesus macaque.

• IHC
• IHC - Frozen
• Immunofluorescence
• Western blot
• Flow Cytometry (1:50 - 1:100)
• ELISA

• IHC - Paraffin

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request.

PBS

Store at -20°C Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P16284 NCBI: NM_000442 NP_000433.4