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Antibodies > PXN / Paxillin > LS-C358273
 
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Catalog Number Size Price
LS-C358273-100 100 µl (1 mg/ml) $299 
PXN / Paxillin Antibody - Immunohistochemical analysis of Paxillin staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
PXN / Paxillin Antibody - Immunofluorescent analysis of Paxillin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
PXN / Paxillin Antibody - Western blot analysis of Paxillin expression in HeLa (A) whole cell lysates.
PXN / Paxillin Antibody - Immunohistochemical analysis of Paxillin staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
PXN / Paxillin Antibody - Immunofluorescent analysis of Paxillin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
PXN / Paxillin Antibody - Western blot analysis of Paxillin expression in HeLa (A) whole cell lysates.
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Polyclonal Rabbit anti‑Human PXN / Paxillin Antibody (N‑Terminus, IHC, IF, WB) LS‑C358273

Polyclonal Rabbit anti‑Human PXN / Paxillin Antibody (N‑Terminus, IHC, IF, WB) LS‑C358273

Submit Review LSBio Guarantee
Antibody:
PXN / Paxillin Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C358273-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
PXN / Paxillin Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
Paxillin antibody LS-C358273 is an unconjugated rabbit polyclonal antibody to Paxillin (PXN) (N-Terminus) from human. It is reactive with human and pig. Validated for ICC, IF, IHC and WB.
Target
Human PXN / Paxillin
Synonyms
PXN | Paxillin
Host
Rabbit
Reactivity
Human, Pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-Terminus of human Paxillin.
Epitope
N-Terminus
Specificity
Recognizes endogenous levels of Paxillin protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PXN / Paxillin
P49023 NM_002859 NP_002850.2

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Featured Products

PXN / Paxillin Antibody - Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with PXN Antibody (Y118) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
PXN / Paxillin Antibody (Tyr118) LS‑C99197
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
PXN / Paxillin Antibody - Immunohistochemical analysis of Paxillin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
PXN / Paxillin Antibody (Internal) LS‑C352762
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Product
PXN / Paxillin Antibody (Tyr118, Biotin) LS‑C220282
Species: Human
Applications: IHC, Western blot, ELISA
Product
PXN / Paxillin Antibody (aa59‑274) LS‑C38105
Species: Human, Mouse, Rat
Applications: Western blot
PXN / Paxillin Antibody - Western blot of extracts from HT-29, using Paxillin (Phospho-Ser178) Antibody.
PXN / Paxillin Antibody (phospho‑Ser178) LS‑C415714
Species: Human, Mouse, Rat
Applications: Western blot, ELISA
PXN / Paxillin Protein
Human PXN / Paxillin Protein (Recombinant 6His, N-terminus) (Asp2-Cys557) - LS-G26507
Source: E. coli
Tag: 6His, N-terminus
See all PXN / Paxillin Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/22/2024
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For technical assistance:
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