Human TP53 / p53

Mouse

Human (tested or 100% immunogen sequence identity)

Mouse, Rat

IgG2a Monoclonal

DO-1

Unconjugated

Protein G purified

Unmodified

Recombinant human p53 (AA 20-25)

aa20-25

Recognizes the human p53 tumor suppressor protein, also known as cellular tumor antigen p53 or NY-CO-13. Clone DO-1 binds to both wild type and mutant forms of the p53 protein found in various malignancies. p53 is important in multicellular organisms, where it regulates cell cycle progression to allow DNA repair or apoptosis in the case of irreparably damaged cells and thus functions as a tumor suppressor that is involved in preventing cancer. Mutations in the p53 gene are found in about half the cases of human cancerrecognizes an epitope at the N-terminal end of p53 between amino acids 20-25, common to isoforms 1-3 of p53.

• IHC
• IHC - Paraffin (1:25)
• IHC - Frozen
• Western blot (1:1000)
• Immunoprecipitation
• ELISA

Immunohistology: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. We recommend the use of 0.01 M citrate buffer for this purpose.

PBS, 0.09% Sodium Azide

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P04637 NCBI: NM_000546 NP_000537.3