Human VEGFA / VEGF

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

Unconjugated

Protein A purified

Unmodified

E.coli derived recombinant Human VEGF165.

Human VEGF

• Western blot (0.25 - 0.5 µg/ml)
• ELISA (2 - 4 µg/ml)

Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human VEGF (10 ng/ml), a concentration of &le. 0.2 ug/ml of this antibody is required. ELISA: In a sandwich ELISA (assuming 100ul/well), a concentration of 2-4 ug/ml of this antibody will detect at least 100 pg/ml of recombinant human VEGF165 when used with biotinylated antigen affinity purified anti-human VEGF165 (LS-C104615) as the detection antibody at a concentration of approximately 0.25-1 ug/ml. Western Blot: To detect hVEGF165 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF165 is 0.25-0.50 ng/lane, under non-reducing conditions.

Lyophilized from PBS

Reconstitute in sterile water to 1 mg/ml.

Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P15692 NCBI: NM_003376 NP_003367.4