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Catalog Number Size Price
LS-K308-100 100 Tests $450 
GPT / Alanine Transaminase Assay Kit

Alanine Transaminase (ALT) Assay Kit (Colorimetric) - LS-K308

Alanine Transaminase (ALT) Assay Kit (Colorimetric) - LS-K308

Available for shipment within the USA only
Description:
Alanine Transaminase (ALT), also known as serum alanine aminotransferase (ALAT) or pyruvic transaminase (SGPT), facilitates the conversion of alanine and a-ketoglutarate to pyruvate and glutamate. ALT plays an important role in gluconeogenesis and amino acid metabolism. ALT is found mainly in the liver, and, to a lesser extent, in kidney, heart, muscle, and pancreas tissues. Normal serum levels of ALT are low, and increased serum ALT activity is widely used as a marker for liver damage. Simple, direct and automation-ready procedures for measuring ALT activity find wide applications in research and drug discovery. This ALT activity assay is based on the quantification of pyruvate produced by ALT. In this assay, pyruvate and NADH are converted to lactate and NAD by the enzyme lactate dehydrogenase (LDH). The decrease in NADH absorbance at 340 nm is proportional to ALT activity.
Price
Catalog Number
$450
LS-K308-100

Available for USA Shipment Only
Toll Free North America
206-374-1102
For Research Use Only

Overview

Description:
Alanine Transaminase (ALT), also known as serum alanine aminotransferase (ALAT) or pyruvic transaminase (SGPT), facilitates the conversion of alanine and a-ketoglutarate to pyruvate and glutamate. ALT plays an important role in gluconeogenesis and amino acid metabolism. ALT is found mainly in the liver, and, to a lesser extent, in kidney, heart, muscle, and pancreas tissues. Normal serum levels of ALT are low, and increased serum ALT activity is widely used as a marker for liver damage. Simple, direct and automation-ready procedures for measuring ALT activity find wide applications in research and drug discovery. This ALT activity assay is based on the quantification of pyruvate produced by ALT. In this assay, pyruvate and NADH are converted to lactate and NAD by the enzyme lactate dehydrogenase (LDH). The decrease in NADH absorbance at 340 nm is proportional to ALT activity.

Specifications

Name
Alanine Transaminase (ALT) Assay Kit (Colorimetric)
Type
Detection/Quantition
Usage
For quantitative determination of alanine transaminase activity.
Target
GPT / Alanine Transaminase
Synonyms
GPT | AAT1 | ALT1 | Alanine aminotransferase | Alanine aminotransferase 1 | GPT 1 | GPT1
SampleType
Plasma, Serum
Detection
Colorimetric (340 nm Absorbance)
Supplied Components
The following components are supplied with this product.
  • Assay Buffer
  • LDH
  • Cosubstrate
  • NADH Reagent: Dried
  • (See Datasheet for specific volumes supplied)
Applications
Spectrophotometry (visible)
Equipment
Microplate spectrophotometer (visible), Spectrophotometer (visible)
Conditions
Shipped -20°C Dry Ice, Store at -20°C, 3 months shelf life.
Documents
Restrictions
For research use only. Intended for use by laboratory professionals. Available for shipment within the USA only
Guarantee
This Assay Kit carries the LSBio 100% Guarantee

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Images

Colorimetry

GPT / Alanine Transaminase Assay Kit

Colorimetry

GPT / Alanine Transaminase Assay Kit

Colorimetry

GPT / Alanine Transaminase Assay Kit

Colorimetry

GPT / Alanine Transaminase Assay Kit

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 6/16/2024