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| Catalog Number | Size | Price |
|---|---|---|
| LS-B16617-50 | 50 µg (0.2 mg/ml) | $460 |
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PathPlus™ Monoclonal Mouse anti‑Human BCL2 / Bcl‑2 Antibody (clone 124, aa41‑54, IHC, IF, WB) LS‑B16617
PathPlus™ Monoclonal Mouse anti‑Human BCL2 / Bcl‑2 Antibody (clone 124, aa41‑54, IHC, IF, WB) LS‑B16617
Note: This antibody replaces LS-C357724
Antibody:
BCL2 / Bcl-2 Mouse anti-Human Monoclonal (aa41-54) (124) Antibody
Application:
IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
BCL2 / Bcl-2 Mouse anti-Human Monoclonal (aa41-54) (124) Antibody
Application:
IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Specifications
Description
BCL2 is a regulator of apoptosis that functions to promote cell survival and inhibit the actions of proteins involved in the apoptosis cascade. Overexpression of BCL2 has an oncogenic effect and is correlated with increasing the lifespan of B cells. Cancers that are positive include follicular lymphomas due to t(14,18)(q32;q21), which brings BCL2 gene adjacent to the active immunoglobulin heavy chain (IgH) gene. In addition, positive staining is expected in diffuse large B-cell cell lymphomas and myelodysplastic syndrome. In normal tissues, positive expression is found in mantle zone B cells, germinal center T cells, and several other tissues.
References: Cell Death and Differentiation 25:65, 2018; Am J Surg Pathol 2011;35:1691, Am J Surg Pathol 2009;33:591
Target
Human BCL2 / Bcl-2
Synonyms
BCL2 | Apoptosis regulator Bcl-2 | B-cell CLL/lymphoma 2 | B-cell lymphoma protein 2 | Bcl-2 | PPP1R50
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Non-Reactivity
Mouse, Rat
Clonality
IgG1,k
Monoclonal
Clone
124
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified.
Also available Ready-to-Use, Azide-free, BSA-free.
Immunogen
A synthetic peptide, aa 41-54 (G aa PAPGIFSSQPG-Cys) of human Bcl-2 protein. Percent identity by BLAST analysis: Human, Chimpanzee, Cat, Pig, Dog (100%); Gorilla, Orangutan, Bovine, Goat (91%); Galago (82%).
Epitope
aa41-54
Specificity
Human BCL2 / Bcl-2
Applications
- IHC - Paraffin (1:50)
- Immunofluorescence
- Western blot
- Flow Cytometry
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
This antibody recognizes a protein of 25-26kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of Bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express Bcl-2 protein and the small number in which the neoplastic cells are Bcl-2 negative. ELISA: Order BSA-free format for coating.
Presentation
1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide
Storage
Store at 2°C to 8°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About BCL2 / Bcl-2
Validation
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
LSBio Ratings
PathPlus™ BCL2 / Bcl-2 Antibody (aa41-54, clone 124) for IHC, IF/Immunofluorescence, WB/Western, Flow LS-B16617 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Species:
Human
Applications:
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Species:
Human
Applications:
IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/21/2024
Date Requested: 11/21/2024
