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Catalog Number	Size	Price
LS-B16222-100	100 µg (200 µg/ml)	$525

BCL2 / Bcl-2 Antibody - Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)

BCL2 / Bcl-2 Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

BCL2 / Bcl-2 Antibody - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

BCL2 / Bcl-2 Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

BCL2 / Bcl-2 Antibody - Western Blot Analysis of MCF-7 Cell lysate using Bcl-2 Mouse Recombinant Monoclonal Antibody (rBCL2/782).

BCL2 / Bcl-2 Antibody - SDS-PAGE Analysis Purified Bcl-2 Mouse Recombinant Monoclonal Antibody (rBCL2/782). Confirmation of Purity and Integrity of Antibody.

BCL2 / Bcl-2 Antibody - Analysis of Protein Array containing more than 19,000 full-length human proteins using Bcl-2 Mouse Recombinant Monoclonal Antibody (rBCL2/782).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

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PathPlus™ Recombinant Monoclonal Mouse anti‑Human BCL2 / Bcl‑2 Antibody (clone rBCL2/782, IHC, IF, WB) LS‑B16222

Name: PathPlus™ BCL2 / Bcl-2 Recombinant Monoclonal Antibody Mouse | LSBio
Brand: Lifespan BioSciences
SKU: LS-B16222-100
Price: 525.0000 USD
Availability: InStock
Rating: 5 (1 reviews)

Cancer Pathology Cancer

(5.0) Rating

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Note: This antibody replaces LS-C751338

Antibody:

BCL2 / Bcl-2 Mouse anti-Human Recombinant Monoclonal (rBCL2/782) Antibody

Application:

IHC-P, IF, WB, Flo

Reactivity:

Human

Format:

Unconjugated, Unmodified

Other formats:

Azide-free, Carrier-free

$525

LS-B16222-100

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

BCL2 / Bcl-2 Mouse anti-Human Recombinant Monoclonal (rBCL2/782) Antibody

Application:

IHC-P, IF, WB, Flo

Reactivity:

Human

Format:

Unconjugated, Unmodified

Other formats:

Azide-free, Carrier-free

Specifications

Description

BCL2 is a regulator of apoptosis that functions to promote cell survival and inhibit the actions of proteins involved in the apoptosis cascade. Overexpression of BCL2 has an oncogenic effect and is correlated with increasing the lifespan of B cells. Cancers that are positive include follicular lymphomas due to t(14,18)(q32;q21), which brings BCL2 gene adjacent to the active immunoglobulin heavy chain (IgH) gene. In addition, positive staining is expected in diffuse large B-cell cell lymphomas and myelodysplastic syndrome. In normal tissues, positive expression is found in mantle zone B cells, germinal center T cells, and several other tissues. References: Cell Death and Differentiation 25:65, 2018; Am J Surg Pathol 2011;35:1691, Am J Surg Pathol 2009;33:591

Target

Human BCL2 / Bcl-2

Synonyms

See All BCL2 / Bcl-2 Antibodies

Host

Mouse

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

IgG1,k Recombinant Monoclonal

Clone

rBCL2/782

Conjugations

Unconjugated

Purification

Protein A/G purified

Modifications

Unmodified. Also available Azide-free, Carrier-free.

Immunogen

Recombinant full-length human bcl-2 protein. Antigen Molecular Weight: 25-26 kDa

Specificity

This antibody recognizes a protein of 25-26 kDa , identified as the bcl-2alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.

Applications

IHC - Paraffin (1:50)
Immunofluorescence (1 - 2 µg/ml)
Western blot (1 - 2 µg/ml)
Flow Cytometry (1 - 2 µg/10E6 cells)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Western Blotting (0.5-1 ug/ml) - Immunohistology (Formalin-fixed - 0.5-1 ug/ml for 30 minutes at RT - Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA Buffer, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.) Optimal dilution for a specific application should be determined.

Presentation

10 mM PBS, 0.05% BSA, 0.05% Sodium Azide

Storage

Store at 2°C to 8°C for up to 2 years.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About BCL2 / Bcl-2

Uniprot: P10415 NCBI: NM_000633 NP_000624.2