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| Catalog Number | Size | Price |
|---|---|---|
| LS-A4271-50 | 50 µg (1 mg/ml) | $440 |
PathPlus™ Polyclonal Rabbit anti‑Human GPER1 / GPR30 Antibody (Extracellular Domain, IHC, IF, WB) LS‑A4271
PathPlus™ Polyclonal Rabbit anti‑Human GPER1 / GPR30 Antibody (Extracellular Domain, IHC, IF, WB) LS‑A4271
Note: This antibody replaces LS-C66914
Antibody:
GPER1 / GPR30 Rabbit anti-Human Polyclonal (Extracellular Domain) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Overview
Antibody:
GPER1 / GPR30 Rabbit anti-Human Polyclonal (Extracellular Domain) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Specifications
Description
G-protein coupled estrogen receptor 1 (GPER1) promotes neuritogenesis in developing hippocampal neurons. It plays a role in acute neuroprotection against NMDA-induced excitotoxic neuronal death and increases firing activity and intracellular calcium oscillations in luteinizing hormone-releasing hormone (LHRH) neurons. GPER1 inhibits mature adipocyte differentiation and lipid accumulation, and is involved in the recruitment of beta-arrestin 2 ARRB2 at the plasma membrane in epithelial cells. It also functions as a receptor for aldosterone, mediating rapid regulation of vascular contractibility through the PI3K/ERK signaling pathway. Finally, it is implicated in cancer progression.
References: The UniProt Consortium. Nucleic Acids Res. 47: D506-515 (2019); Nucleic Acids Res. 2016 Jan 4;44(D1):D733-45, PMID:26553804
Target
Human GPER1 / GPR30
Synonyms
GPER1 | CMKRL2 | CEPR | FEG-1 | G-protein coupled receptor 30 | G protein-coupled receptor 30 | GPCR-Br | GPER | Heptahelix receptor | LERGU | LyGPR | MER | GPR30 | IL8-related receptor DRY12 | LERGU2 | Chemokine receptor-like 2 | DRY12 | Membrane estrogen receptor
Host
Rabbit
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Dog, Hamster (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic 16 amino acid peptide from 3rd extracellular domain of human GPR30. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%); Mouse, Rat, Hamster, Elephant, Dog, Opossum (94%); Panda, Bat, Horse, Rabbit, Platypus (88%).
Epitope
Extracellular Domain
Specificity
Human GPR30. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Applications
- IHC
- IHC - Paraffin (6.4 µg/ml)
- Immunofluorescence
- Western blot
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Antibody does not work for Western blot with mouse samples.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About GPER1 / GPR30
Validation
Anti-GPR30 antibody IHC of human brain, hippocampus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR30 antibody IHC of human brain, hippocampus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
See More About...
LSBio Ratings
PathPlus™ GPER1 / GPR30 Antibody (Extracellular Domain) for IHC, IF/Immunofluorescence, WB/Western LS-A4271 has an LSBio Rating of
Publications (4.7)
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (8)
Identification of the estrogen receptor GPER in neoplastic and non-neoplastic human testes. Rago V, Romeo F, Giordano F, Maggiolini M, Carpino A. Reproductive biology and endocrinology : RB&E. 2011 9:135. (WB, IHC-P; Human)
Nuclear alternate estrogen receptor GPR30 mediates 17beta-estradiol-induced gene expression and migration in breast cancer-associated fibroblasts. Madeo A, Maggiolini M. Cancer research. 2010 Jul;70:6036-46.
Identification of G protein-coupled estrogen receptor in human and pig spermatozoa. Rago V, Giordano F, Brunelli E, Zito D, Aquila S, Carpino A. Journal of anatomy. 2014 Jun;224:732-6.
Expression pattern of G protein-coupled receptor 30 in LacZ reporter mice. Isensee J, Meoli L, Zazzu V, Nabzdyk C, Witt H, Soewarto D, Effertz K, Fuchs H, Gailus-Durner V, Busch D, Adler T, de Angelis MH, Irgang M, Otto C, Noppinger PR. Endocrinology. 2009 Apr;150:1722-30.
G protein-coupled receptor 30 expression is up-regulated by EGF and TGF alpha in estrogen receptor alpha-positive cancer cells. Vivacqua A, Lappano R, De Marco P, Sisci D, Aquila S, De Amicis F, Fuqua SA, And[Character f2] S, Maggiolini M. Molecular endocrinology (Baltimore, Md.). 2009 Nov;23:1815-26.
c-Jun activation is required for 4-hydroxytamoxifen-induced cell death in breast cancer cells. Madeo A, Vinciguerra M, Lappano R, Galgani M, Gasperi-Campani A, Maggiolini M, Musti AM. Oncogene. 2010 Feb;29:978-91.
Stallion spermatozoa: putative target of estrogens; presence of the estrogen receptors ESR1, ESR2 and identification of the estrogen-membrane receptor GPER. Arkoun B, Gautier C, Delalande C, Barrier-Battut I, Gu[Character e9]non I, Goux D, Boura[Character ef]ma-Lelong H. General and comparative endocrinology. 2014 May;200:35-43.
Estriol acts as a GPR30 antagonist in estrogen receptor-negative breast cancer cells. Lappano R, Rosano C, De Marco P, De Francesco EM, Pezzi V, Maggiolini M. Molecular and cellular endocrinology. 2010 May;320:162-70.
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Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, ELISA
Species:
Human, Monkey, Rat, Dog
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, ICC, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence
Species:
Human, Monkey, Dog
Applications:
IHC, IHC - Paraffin
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/20/2024
Date Requested: 12/20/2024
