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| Catalog Number | Size | Price |
|---|---|---|
| LS-B4244-50 | 50 µg (2 mg/ml) | $565 |
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PathPlus™ Polyclonal Sheep anti‑Human HNE / Neutrophil Elastase Antibody (IHC) LS‑B4244
PathPlus™ Polyclonal Sheep anti‑Human HNE / Neutrophil Elastase Antibody (IHC) LS‑B4244
Note: This antibody replaces LS-C23010
Antibody:
HNE / Neutrophil Elastase Sheep anti-Human Polyclonal Antibody
Application:
IHC-P, ELISA, IE
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
HNE / Neutrophil Elastase Sheep anti-Human Polyclonal Antibody
Application:
IHC-P, ELISA, IE
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
ELANE (HNE, Neutrophil Elastase) is a serine protease that hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. It is thought to participate in degenerative and inflammatory diseases via its proteolysis of collagen-IV and elastin of the extracellular matrix. ELANE mutations are correlated with cyclic neutropenia and severe congenital neutropenia (SCN). In immunohistochemistry, ELANE has cytoplasmic positivity in neutrophils and bone marrow cells.
References: The UniProt Consortium. Nucleic Acids Res. 47: D506-515 (2019); Nucleic Acids Res. 2016 Jan 4;44(D1):D733-45, PMID:26553804; BMC Hematol 15, 2 (2015), DOI:10.1186/s12878-015-0020-x
Target
Human HNE / Neutrophil Elastase
Synonyms
ELANE | Bone marrow serine protease | ELA2 | Elastase 2, neutrophil | Elastase-2 | HLE | Medullasin | Granulocyte-derived elastase | Neutrophil elastase | NE | Human leukocyte elastase | PMN elastase | PMN-E | SCN1 | Polymorphonuclear elastase | Elastase, neutrophil expressed | GE | Leukocyte elastase | Lysosomal elastase | HNE
Host
Sheep
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Ion exchange chromatography
Modifications
Unmodified
Immunogen
Neutrophil elastase (active-site blocked) prepared from human sputum.
Specificity
Specific for elastase.
Applications
- IHC - Paraffin (5 µg/ml)
- ELISA
- Immunoelectrophoresis
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Suitable for use in ELISA, IHC with FFPE tissues and Immunoelectrophoresis.
Presentation
10 mM HEPES, pH 7.4, 0.15 M NaCl, 50% Glycerol
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About HNE / Neutrophil Elastase
Validation
Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
LSBio Ratings
PathPlus™ HNE / Neutrophil Elastase Antibody for IHC, ELISA LS-B4244 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Intrarenal Toll-like receptor 4 and Toll-like receptor 2 expression correlates with injury in antineutrophil cytoplasmic antibody-associated vasculitis. O'Sullivan KM, Ford SL, Longano A, Kitching AR, Holdsworth SR. American journal of physiology. Renal physiology. 2018 November;315:F1283-F1294.
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC - Paraffin, ELISA
Species:
Human
Applications:
ELISA, Immunoelectrophoresis
Species:
Human
Applications:
Flow Cytometry, ELISA
Species:
Human
Applications:
Western blot, ELISA
Species:
Human
Applications:
Western blot, ELISA
Species:
Human
Applications:
ELISA, Enzyme Kinetics, Inhibition
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
