Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Forgot password?
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Quick Order
Products
Antibodies
ELISA and Assay Kits
Research Areas
Infectious Disease
Resources
Purchasing
Reference Material
Contact Us
Location
Corporate Headquarters
Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States
Telephone Numbers
Customer Service: (800) 227-6666 / (650) 697-3600
Contact Us
Additional Contact Details
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Forgot password?
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Quick Order
| Catalog Number | Size | Price |
|---|---|---|
| LS-B5426-50 | 50 µg (0.5 mg/ml) | $440 |
![KRT4 / CK4 / Cytokeratin 4 Antibody - Immunofluorescence of monoclonal antibody to KRT4 on A-431 cell. [antibody concentration 10 ug/ml]](https://lsbio-7d62.kxcdn.com/image2/pathplus-krt4-ck4-cytokeratin-4-antibody-clone-5h5-ls-b5426/51058_47015.jpg)
1 of 8
2 of 8
3 of 8
4 of 8
5 of 8
6 of 8
7 of 8
8 of 8
PathPlus™ Monoclonal Mouse anti‑Human KRT4 / CK4 / Cytokeratin 4 Antibody (clone 5H5, IHC, IF, WB) LS‑B5426
PathPlus™ Monoclonal Mouse anti‑Human KRT4 / CK4 / Cytokeratin 4 Antibody (clone 5H5, IHC, IF, WB) LS‑B5426
Note: This antibody replaces LS-C133283
Antibody:
KRT4 / CK4 / Cytokeratin 4 Mouse anti-Human Monoclonal (5H5) Antibody
Application:
IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
KRT4 / CK4 / Cytokeratin 4 Mouse anti-Human Monoclonal (5H5) Antibody
Application:
IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
KRT4 (Cytokeratin 4) is a member of the keratin gene family, a type II cytokeratin that is specifically expressed in differentiated layers of the mucosal and esophageal epithelia along with KRT13. Mutations in KRT4 and KRT13 are associated with White Sponge Nevus, described by oral, esophageal, and anal leukoplakia. In immunohistochemistry, KRT4 has cytoplasmic positivity in suprabasal cells of non-keratinized, stratified squamous epithelium of the esophagus and cornea, airway epithelia, and it is also expressed in the anus, larynx, pharynx, and tongue.
References: The UniProt Consortium. Nucleic Acids Res. 47: D506-515 (2019); Nucleic Acids Res. 2016 Jan 4;44(D1):D733-45, PMID:26553804; J Biol Chem 1998;273:23912, PMID: 9727005; Meta Gene. Volume 2, December 2014: 374-383, DOI: 10.1016/j.mgene.2014.04.008
Target
Human KRT4 / CK4 / Cytokeratin 4
Synonyms
KRT4 | CK-4 | CK4 | Cytokeratin 4 | Cytokeratin-4 | K4 | Keratin 4 | Type-II keratin Kb4 | CYK4 | Keratin-4
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Clone
5H5
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Immunogen
KRT4 (NP_002263, 194 aa ~ 301 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human Keratin 4
Applications
- IHC - Paraffin (5 µg/ml)
- Immunofluorescence (10 µg/ml)
- Western blot (1:500 - 1:1000)
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using cell lysates and the recombinant protein used as the immunogen.
Presentation
PBS, pH 7.4
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About KRT4 / CK4 / Cytokeratin 4
Validation
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Anti-KRT4 / Cytokeratin 4 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-KRT4 / Cytokeratin 4 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
LSBio Ratings
PathPlus™ KRT4 / CK4 / Cytokeratin 4 Antibody (clone 5H5) for IHC, IF/Immunofluorescence, WB/Western, ELISA LS-B5426 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot
Reactivity:
Human
Range:
0.156-10 ng/ml
Source:
E. coli
Tag:
His-GST
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
