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Catalog Number	Size	Price
LS-B391-100	100 µg (0.98 mg/ml)	$505

MCM2 Antibody - Anti-MCM2 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

MCM2 Antibody - Anti-MCM2 Antibody - Western Blot. Western blot of Affinity Purified anti-MCM2 antibody shows detection of both phosphorylated and unphosphorylated MCM2 present in nuclear extracts from elutriated human cells (MO59K/K562). The MCM2 protein is phosphorylated after initiation of DNA replication, therefore, the protein is unphosphorylated in early S phase, and gradually becomes phosphorylated throughout S phase. In G2/M, all MCM2 is phosphorylated. Panel A shows western blot results for lysates were prepared from asynchronous cells (lane 1), cells arrested in early S with aphidicolin (lane2), and cells arrested in mitosis with nocodazole (lane 3). Panel B shows a schematic diagram of bands representing phosphorylated and unphosphorylated MCM2 present in these preparations. Asynchronous cells contain a doublet of both forms. Aphidicolin treated cells contain only unphosphorylated MCM2 and nocodozole treatment results in only phosphorylated MCM2 detected in the lysate. The phosphorylated band migrates faster than the unphosphorylated form and is seen as the lower band. There is a clear switch from the unphosphorylated form in the center lane, to the phosphorylated form in the third lane, confirming recognition of both forms of MCM2 by this antibody. The primary antibody was diluted 1:400 for this experiment. Personal Communication, Jennifer Seiler, NIH, CCR, Bethesda, MD.

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PathPlus™ Polyclonal Rabbit anti‑Human MCM2 Antibody (aa15‑40, IHC, WB) LS‑B391

Name: PathPlus™ MCM2 Polyclonal Antibody Rabbit anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-B391-100
Price: 505.0000 USD
Availability: InStock
Rating: 5 (1 reviews)

Cancer

(5.0) Rating

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Note: This antibody replaces LS-C18788

Antibody:

MCM2 Rabbit anti-Human Polyclonal (aa15-40) Antibody

Application:

IHC, IHC-P, WB, ELISA

Reactivity:

Human, Mouse, Rat, S. cerevisiae

Format:

Unconjugated, Unmodified

$505

LS-B391-100

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

MCM2 Rabbit anti-Human Polyclonal (aa15-40) Antibody

Application:

IHC, IHC-P, WB, ELISA

Reactivity:

Human, Mouse, Rat, S. cerevisiae

Format:

Unconjugated, Unmodified

Specifications

Description

MCM2 is a component of the MCM complex, a replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. MCM2 is required for entry into the S phase and for cell division. MCM2 is a useful marker in immunohistochemistry for characterizing a number of cancers. It is overexpressed in precancerous and cancerous cervical lesions and upregulation is associated with high-risk type HPV. In squamous cell and adenocarcinoma of the gallbladder, MCM2 positivity is associated with metastasis and poor prognosis. It has similar prognostic value in oral dysplasia and ovarian serous neoplasms, where expression increases with severity of tumor grade. In immunohistochemistry of normal tissue, MCM2 has nuclear positivity in proliferative cells throughout the body. References: Int J Clin Exp Pathol. 2015; 8(1): 875–880, PMID: 25755789; Mol Med Rep. 2016 Nov; 14(5): 4581–4592, PMID: 27748889; Applied Immunohistochemistry & Molecular Morphology. 2018 Aug. 26(7):509–513, DOI: 10.1097/PAI.0000000000000330; British Journal of Cancer. 2004. 90:1583–1590, PMID: 15083189

(1) Publication(s)

Target

Human MCM2

Synonyms

See All MCM2 Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat, S. cerevisiae (tested or 100% immunogen sequence identity)

Predicted

Human, Mouse, Rat, S. cerevisiae (at least 90% immunogen sequence identity)

Clonality

IgG Polyclonal

Conjugations

Unconjugated

Purification

Affinity purified

Modifications

Unmodified

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an N-Terminal region near amino acids 15-40 of human MCM2 protein.

Epitope

aa15-40

Specificity

Approximately equivalent reactivity occurs against both unphosphorylated and phosphorylated forms of human MCM2 (phosphorylated at residues pS26 and pS27). A BLAST analysis was used to suggest cross reactivity with MCM2 proteins from human, mouse, rat and S. cerevisiae based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Applications

IHC
IHC - Paraffin (5 µg/ml)
Western blot (1:500 - 1:2000)
ELISA (1:10000 - 1:50000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Immunohistochemistry: LS-B391 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B391 was determined to be 5 ug/ml.

Presentation

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Storage

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About MCM2

Uniprot: P49736 NCBI: NM_004526 NP_004517.2

Validation

Anti-MCM2 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

See More About...

PathPlus™ Validation Process

LSBio Ratings

PathPlus™ MCM2 Antibody (aa15-40) for IHC, WB/Western, ELISA LS-B391 has an LSBio Rating of

(5.0) Rating

Publications (4)

Laboratory Validation Score (5)

Learn more about The LSBio Ratings Algorithm

Publications (1)

The opposing transcriptional functions of Sin3a and c-Myc are required to maintain tissue homeostasis. Nascimento EM, Cox CL, MacArthur S, Hussain S, Trotter M, Blanco S, Suraj M, Nichols J, Kbler B, Benitah SA, Hendrich B, Odom DT, Frye M. Nature cell biology. 2011 13:1395-405. (IHC-P; Mouse)

Full Text Article PubMed: 22101514 PMC: PMC3242072

Submit a Publication for LS-B391