Human NANOG

Rabbit

Human (tested or 100% immunogen sequence identity)

Monkey, Cow (at least 90% immunogen sequence identity)

Polyclonal

Unconjugated

Ammonium sulfate precipitation

Unmodified

aa94-123

This NANOG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 94-123 amino acids from the Central region of human NANOG.

• IHC
• IHC - Paraffin (1:25)
• Immunofluorescence (1:10 - 1:50)
• Western blot (1:1000)
• Flow Cytometry (1:10 - 1:50)

Immunohistochemistry: LS-B3425 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3425 was determined to be 10 ug/ml.

PBS, 0.09% Sodium Azide

Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9H9S0 NCBI: AB062943 NP_079141.2