Human SAA1 / SAA / Serum Amyloid A

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

Unconjugated

Protein G purified

Unmodified

Highly purified recombinant human SAA, 12kD

Recognizes natural and recombinant human SAA. Does not show any cross-reactivity with other human cytokines or growth factors tested such as IL-1b, IL-8, MCAF, TGF-b and EGF.

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot
• ELISA (0.3 µg/ml)
• Sandwich ELISA

Immunohistochemistry: LS-B2130 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2130 was determined to be 5 ug/ml. ELISA (capture coating): 0.3 ug/ml Detects 1.1 ng/ml of SAA in serum/plasma or 0.6 ng/ml of SAA in medium with an assay range of 0 to 80 ng/ml. Suggested substrate: TMB. Western Blot: 0.1-0.5 ug allows visualization of 100 ng/lane of recombinant human SAA. Used in sandwich ELISA as the capture antibody with LS-B2208 as the detection antibody.

PBS, pH 7.0

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P02735 NCBI: NM_000331 NP_000322.2