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| Catalog Number | Size | Price |
|---|---|---|
| LS-B16203-0.05 | 0.05 ml | $460 |
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PathPlus™ Monoclonal Mouse anti‑Human SDC1 / Syndecan 1 / CD138 Antibody (Concentrated, IHC) LS‑B16203
PathPlus™ Monoclonal Mouse anti‑Human SDC1 / Syndecan 1 / CD138 Antibody (Concentrated, IHC) LS‑B16203
Note: This antibody replaces LS-C757332
Antibody:
SDC1 / Syndecan 1 / CD138 Mouse anti-Human Monoclonal (Concentrated) Antibody
Application:
IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Concentrated
Other formats:
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
SDC1 / Syndecan 1 / CD138 Mouse anti-Human Monoclonal (Concentrated) Antibody
Application:
IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Concentrated
Other formats:
Specifications
Description
Syndecan 1 (SDC1, CD138) is a cell surface heparin sulfate proteoglycan that links the cytoskeleton to the interstitial matrix. It mediates cell binding, cell signaling, cytoskeletal organization, and participates in cell proliferation and cell migration. It is also involved in internalization of the HIV-1 tat protein. Altered SDC1 expression has been detected in several cancers, including multiple myeloma. It is expressed in plasma cells, squamous epithelia of the esophagus and skin, placental trophoblasts, and a variety of epithelial cells including those of the prostate, GI tract and breast.
References: Ref: Am J Clin Path 2004, 121(2):254; Adv Exp Med Biol 1992, 313:69
Target
Human SDC1 / Syndecan 1 / CD138
Synonyms
SDC1 | CD138 antigen | SDC | SYND1 | Syndecan | Syndecan 1 | Syndecan-1 | CD138 | Syndecan proteoglycan 1
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
Monoclonal
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Concentrated.
Also available Ready-to-Use.
Immunogen
Recombinant human CD138.
Applications
- IHC - Paraffin (1:200)
- ELISA (1:100 - 1:200)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Dilute antibody to its recommended working dilution using the same buffer defined in the Presentation. Manual IHC Protocol: 1) Perform Heat-Induced Epitope Retrieval at pH 9 for 10 to 30 minutes. 2) Block in peroxidase blocking solution for 5 minutes at room temperature. (Not required if using Alkaline Phosphatase System) 3) Dilute antibody 1:100-1:200 before applying. Incubate antibody for 10 to 30 minutes at room temperature. 4) Add secondary antibody and incubate for 20 to 30 minutes at room temperature. 5) Add DAB or Fast Red and incubate for 5 to 10 minutes at room temperature. 6) Counterstain with hematoxylin for 0.5 to 5 minutes, depending on the hematoxylin used. Rinse with distilled water and blueing solution for 30 seconds. Dehydrate and apply coverslip.
Presentation
Tris, pH 7.3-7.7, 1% BSA, <0.1% Sodium Azide
Storage
Store at 2°C to 8°C. Do not freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
About SDC1 / Syndecan 1 / CD138
Validation
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
LSBio Ratings
PathPlus™ SDC1 / Syndecan 1 / CD138 Antibody (Concentrated) for IHC, ELISA LS-B16203 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
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Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot, Flow Cytometry
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot, Flow Cytometry
Species:
Human
Applications:
Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
