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Catalog Number	Size	Price
LS-B16260-100	100 µg (200 µg/ml)	$525

TP53 / p53 Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

TP53 / p53 Antibody - Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)

TP53 / p53 Antibody - SDS-PAGE Analysis Purified p53 Mouse Recombinant Monoclonal Antibody (rTP53/1739). Confirmation of Purity and Integrity of Antibody.

TP53 / p53 Antibody - Analysis of Protein Array containing more than 19,000 full-length human proteins using p53 Mouse Monoclonal Antibody (rTP53/1739).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

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PathPlus™ Recombinant Monoclonal Mouse anti‑Human TP53 / p53 Antibody (clone rTP53/1739, IHC, WB) LS‑B16260

Name: PathPlus™ TP53 / p53 Recombinant Monoclonal Antibody Mouse IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-B16260-100
Price: 525.0000 USD
Availability: InStock
Rating: 5 (1 reviews)

Cancer Pathology Cancer

(5.0) Rating

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Note: This antibody replaces LS-C751398

Antibody:

TP53 / p53 Mouse anti-Human Recombinant Monoclonal (rTP53/1739) Antibody

Application:

IHC-P, WB

Reactivity:

Human

Format:

Unconjugated, Unmodified

Other formats:

Azide-free, Carrier-free

$525

LS-B16260-100

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

TP53 / p53 Mouse anti-Human Recombinant Monoclonal (rTP53/1739) Antibody

Application:

IHC-P, WB

Reactivity:

Human

Format:

Unconjugated, Unmodified

Other formats:

Azide-free, Carrier-free

Specifications

Description

TP53 is a tumor suppressor, repair gene and transcriptional regulator, and it is considered the most frequently mutated gene in cancer. Germline mutations in TP53 lead to Li-Fraumeni syndrome and cancer, including early-onset colorectal cancer. Antibodies are used to identify both wild-type and mutant TP53 and its accumulation in various neoplasias, and have strong staining in tumors with mutations that stabilize the protein. TP53 stains positively in breast, lung and endometrial carcinoma, follicular and mantle cell lymphoma, serous ovarian carcinoma, transitional cell carcinoma of the bladder and colorectal adenocarcinoma. TP53 is also often overexpressed in colorectal dysplasia resulting from ulcerative colitis. In normal tissue, TP53 has weak nuclear staining in fractions of cells in most tissues. References: Genome Res. 2017 Oct;27(10):1645-1657, PMID: 28904012; JAMA Oncol. 2015 May;1(2):214-21, PMID: 26086041; Int J Mol Sci. 2017 Jun 16;18(6). pii: E1284, PMID: 28621756

Target

Human TP53 / p53

Synonyms

TP53 | BCC7 | Cellular tumor antigen p53 | p53 | TRP53 | p53 tumor suppressor | Phosphoprotein p53 | Antigen NY-CO-13 | Tumor protein p53 | Tumor suppressor p53 | LFS1

See All TP53 / p53 Antibodies

Host

Mouse

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

IgG1,k Recombinant Monoclonal

Clone

rTP53/1739

Conjugations

Unconjugated

Purification

Protein A/G purified

Modifications

Unmodified. Also available Azide-free, Carrier-free.

Immunogen

Recombinant full-length human TP53 protein. Antigen Molecular Weight: 53 kDa

Specificity

The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. Recognizes a 53 kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53. It is a tumor suppressor protein expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast, lung, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in many malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.

Applications

IHC - Paraffin (1:50)
Western blot (1 - 2 µg/ml)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Immunohistology (Formalin-fixed - 0.5-1.0 ug/ml for 30 minutes at RT - Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.) Optimal dilution for a specific application should be determined.

Presentation

10 mM PBS, 0.05% BSA, 0.05% Sodium Azide

Storage

Store at 2°C to 8°C for up to 2 years.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About TP53 / p53

Uniprot: P04637 NCBI: NM_000546 NP_000537.3