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Catalog Number	Size	Price
LS-A7494-50	50 µg (1 mg/ml)	$460

WNK1 Antibody - Lung, non small cell carcinoma

WNK1 Antibody - Anti-WNK1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

WNK1 Antibody - Anti-WNK1 / p65 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

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PathPlus™ Polyclonal Rabbit anti‑Human WNK1 Antibody (Internal, IHC) LS‑A7494

Name: PathPlus™ WNK1 Polyclonal Antibody Rabbit anti-Human IHC | LSBio
Brand: Lifespan BioSciences
SKU: LS-A7494-50
Price: 460.0000 USD
Availability: InStock
Rating: 5 (1 reviews)

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Antibody:

WNK1 Rabbit anti-Human Polyclonal (Internal) Antibody

Application:

IHC, IHC-P

Reactivity:

Human, Monkey

Format:

Unconjugated, Unmodified

$460

LS-A7494-50

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(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

WNK1 Rabbit anti-Human Polyclonal (Internal) Antibody

Application:

IHC, IHC-P

Reactivity:

Human, Monkey

Format:

Unconjugated, Unmodified

Specifications

Description

Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. References: The UniProt Consortium. Nucleic Acids Res. 47: D506-515 (2019); Nucleic Acids Res. 2016 Jan 4;44(D1):D733-45, PMID:26553804

Target

Human WNK1

Synonyms

See All WNK1 Antibodies

Host

Rabbit

Reactivity

Human, Monkey (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunoaffinity purified

Modifications

Unmodified

Immunogen

Synthetic 19 amino acid peptide from internal region of human WNK1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%); Elephant, Panda (95%); Bat, Rabbit, Pig (89%); Mouse, Rat, Hamster (84%).

Epitope

Internal

Specificity

Human WNK1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Applications

IHC
IHC - Paraffin (7 µg/ml)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 7 ug/ml.

Presentation

PBS, 0.1% Sodium Azide

Storage

Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

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About WNK1

Uniprot: Q9H4A3 NCBI: NM_018979 NP_061852.3

Validation

Brain, Glioblastoma

Lung, non small cell carcinoma

Ovary, carcinoma

Anti-WNK1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-WNK1 / p65 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.