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| Catalog Number | Size | Price |
|---|---|---|
| LS-G118272-100 | 100 µg (1 mg/ml) | $494 |
HNE / Neutrophil Elastase Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G118272
HNE / Neutrophil Elastase Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G118272
Description:
HNE / Neutrophil Elastase Protein LS-G118272 is an Over-Expression Lysate HNE / Neutrophil Elastase with Myc-DDK (Flag) tag(s). For Research Use Only
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Specifications
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Popular HNE / Neutrophil Elastase Proteins
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Overview
Description:
HNE / Neutrophil Elastase Protein LS-G118272 is an Over-Expression Lysate HNE / Neutrophil Elastase with Myc-DDK (Flag) tag(s). For Research Use Only
Specifications
Type
Over-Expression Lysate Protein
Target
HNE / Neutrophil Elastase
Synonyms
ELANE | Bone marrow serine protease | ELA2 | Elastase 2, neutrophil | Elastase-2 | HLE | Medullasin | Granulocyte-derived elastase | Neutrophil elastase | NE | Human leukocyte elastase | PMN elastase | PMN-E | SCN1 | Polymorphonuclear elastase | Elastase, neutrophil expressed | GE | Leukocyte elastase | Lysosomal elastase | HNE
Modifications
Unmodified
Conjugations
Unconjugated
Tag
Myc-DDK (Flag)
Predicted Molecular Weight
28.52 kDa
AA Sequence
MTLGRRLACLFLACVLPALLLGGTALASEIVGGRRARPHAWPFMVSLQLRGGHFCGATLIAPNFVMSAAHCVANVNVRAVRVVLGAHNLSRREPTRQVFAVQRIFENGYDPVNLLNDIVILQLNGSATINANVQVAQLPAQGRRLGNGVQCLAMGWGLLGRNRGIASVLQELNVTVVTSLCRRSNVCTLVRGRQAGVCFGDSGSPLVCNGLIHGIASFVRGGCASGLYPDAFAPVAQFVNWIDSIIQRSEDNPCPHPRDPDPASRTH(CloningSiteandTags:SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV)
Expression System
HEK 293 Cells
Source Species
Human
Purification
Unpurified
Usage Summary
Western blot: Spin down briefly and dilute with 2xSDS Sample Buffer provided (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT). Prior to SDS-PAGE fractionation, boil the lysate for 5-10 minutes.
Bio-Activity
Not Tested
Endotoxin
Not Tested
Presentation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Storage
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
Restrictions
For research use only. Intended for use by laboratory professionals.
About HNE / Neutrophil Elastase
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Images
Western blot
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Western blot
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Western blot
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Western blot
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Popular HNE / Neutrophil Elastase Proteins
Source:
E. coli
Tag:
His-GST
Source:
E. coli
Tag:
6His, N-terminus
Source:
Yeast
Tag:
6His, N-terminus
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
