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| Catalog Number | Size | Price |
|---|---|---|
| LS-G48003-100 | 100 µg | $480 |
| LS-G48003-500 | 500 µg | $1,285 |
Human HMG1 / HMGB1 Protein (Recombinant His,C-terminus) (Met1-Glu215) - LS-G48003
Human HMG1 / HMGB1 Protein (Recombinant His,C-terminus) (Met1-Glu215) - LS-G48003
Description:
HMG1 / HMGB1 Protein LS-G48003 is a Recombinant Human HMG1 / HMGB1 produced in HEK 293 Cells Met1-Glu215 with His,C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
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Overview
Description:
HMG1 / HMGB1 Protein LS-G48003 is a Recombinant Human HMG1 / HMGB1 produced in HEK 293 Cells Met1-Glu215 with His,C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Specifications
Type
Recombinant Protein
Target
HMG1 / HMGB1
Synonyms
HMGB1 | Amphoterin | High mobility group protein B1 | High mobility group box 1 | High mobility group protein 1 | HMG1 | HMG3 | SBP-1 | High-mobility group box 1 | HMG-1
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Applications
- Binding
- ELISA
- Functional Assay
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Tag
His,C-terminus
Region
Met1-Glu215
Predicted Molecular Weight
The recombinant human HMGB1 consists of 226 amino acids and has a predicted molecular mass of 26.3 kDa. As a result of glycosylation, the apparent molecular mass of rhHMGB1 is approximately 30-34 kDa in SDS-PAGE under reducing conditions.
Expression System
HEK 293 Cells
Source Species
Human
Purification
> 93 % as determined by SDS-PAGE
Bio-Activity
Measured by its binding ability in a functional ELISA. Immobilized human HMGB at 2 µg/ml (100 µl/well) can bind human AGER.The EC50 of human AGER is 0.27 µg/ml. Measured by its ability to induce TNF-alpha secretion by RAW 264.7 mouse monocyte/macrophage cells. The EC50 for this effect is typically 20-100µg/ml.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Reconstitute in 400ul of sterile water to a concentration of 0.25mg/ml
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About HMG1 / HMGB1
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Popular HMG1 / HMGB1 Proteins
Source:
E. coli
Tag:
His, N-Terminal
Source:
E. coli
Tag:
His, N-Terminal
Source:
E. coli
Tag:
6His, C-terminus
Source:
Hamster-Chinese
Tag:
Human IgG1 Fc
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/22/2024
Date Requested: 12/22/2024
