Target
THPO / TPO / Thrombopoietin
Synonyms
THPO | C-mpl ligand | MGDF | MPL ligand | THCYT1 | Thrombopoietin nirs variant 1 | MKCSF | MPLLG | Thrombopoietin
Conjugations
Unconjugated
Tag
6His, N-terminus + 6His, C-terminus
Predicted Molecular Weight
75-90 kDa
AA Sequence
HHHHHHSPAPPACDLRVLSKLLRDSHVLHSRLSQCPEVHPLPTPVLLPAVDFSLGEWKTQMEETKAQDILGAVTLLLEGVMAARGQLGPTCLSSLLGQLSGQVRLLLGALQSLLGTQLPPQGRTTAHKDPNAIFLSFQHLLRGKVRFLMLVGGSTLCVRRAPPTTAVPSRTSLVLTLNELPNRTSGLLETNFTASARTTGSGLLKWQQGFRAKIPGLLNQTSRSLDQIPGYLNRIHELLNGTRGLFPGPSRRTLGAPDISSGTSDTGSLPPNLQPGYSPSPTHPPTGQYTLFPLPPTLPTPVVQLHPLLPDPSAPTPTPTSPLLNTSYTHSQNLSQEGVDHHHHHHexpressedwitha6HistagattheN-terminus,and6HistagattheC-terminus
Expression System
Human Cells
Purification
Greater than 95% by SDS-PAGE
Bio-Activity
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells .The ED50 for this effect is typically 0.5-5 ng/mL.
Endotoxin
Less than 1 EU/µg (determined by LAL method).
Presentation
Lyophilized from a 0.2 um filtered solution in 20 mM Tris, pH 8.0, 150 mM NaCl
Reconstitution
Reconstitute in ddH2O at 100 ug/ml.
Storage
Store lyophilized at -70°C or below for up to 6 months. Store reconstituted at 4°C for up to 1 week or at -20°C for up to 3 months. Add a carrier protein (0.1% BSA) for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About THPO / TPO / Thrombopoietin