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Vector Laboratories, Inc.
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Catalog Number Size Price
LS-G136167-1 1 mg (1 mg/ml) $469 
EGF Protein - Western Blot analysis 1 µg recombinant mouse EGF was loaded in lanes 2 and 3, as the primary antibody Goat anti Mouse EGF antibody was used at 0.1 µg/ml and 0.2 µg/ml. HRP conjugated Donkey anti Sheep/Goat IgG antibody was used as the secondary antibody at a dilution of 1/20000 in lanes 2, 3 and 4. Bio-Rad Precision Plus™ Dual Xtra Prestained Protein Standards were run in lane 1. The blot was visualized with the Bio-Rad Chemidoc™ Touch Imaging System.

Mouse EGF Protein (Recombinant) - LS-G136167

Mouse EGF Protein (Recombinant) - LS-G136167

Description:
EGF Protein LS-G136167 is a Recombinant Mouse EGF produced in E. coli. It is biologically active. For Research Use Only
Price
Catalog Number
$469
LS-G136167-1
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Description:
EGF Protein LS-G136167 is a Recombinant Mouse EGF produced in E. coli. It is biologically active. For Research Use Only

Specifications

Type
Recombinant Protein
Target
EGF
Synonyms
EGF | Beta-urogastrone | HOMG4 | URG | Epidermal growth factor | Pro-epidermal growth factor
Species
Mouse
Modifications
Unmodified
Conjugations
Unconjugated
Predicted Molecular Weight
6 kDa
Expression System
E. coli
Source Species
E. coli
Purification
Greater than 98% by HPLC and SDS PAGE
Usage Summary
Recommended applications: Western Blotting, Functional Assays
Bio-Activity
The ED50 as determined by a cell proliferation assay using BALB/c 3T3 cells is = 0.1 ng/ml, corresponding to a specific activity of = 1 x 10^7 units/mg.
Endotoxin
Not Tested
Reconstitution
Reconstitute with H2O to a concentration of 1.0 mg/ml. Gently mix after reconstitution. Do not vortex.
Storage
Store at -20°C. Do not use frost-free freezers. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About EGF
P01133 NM_001963 NP_001954.2

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Images

Western blot

EGF Protein - Western Blot analysis 1 µg recombinant mouse EGF was loaded in lanes 2 and 3, as the primary antibody Goat anti Mouse EGF antibody was used at 0.1 µg/ml and 0.2 µg/ml. HRP conjugated Donkey anti Sheep/Goat IgG antibody was used as the secondary antibody at a dilution of 1/20000 in lanes 2, 3 and 4. Bio-Rad Precision Plus™ Dual Xtra Prestained Protein Standards were run in lane 1. The blot was visualized with the Bio-Rad Chemidoc™ Touch Imaging System.
Western Blot analysis 1 µg recombinant mouse EGF was loaded in lanes 2 and 3, as the primary antibody Goat anti Mouse EGF antibody was used at 0.1 µg/ml and 0.2 µg/ml. HRP conjugated Donkey anti Sheep/Goat IgG antibody was used as the secondary antibody at a dilution of 1/20000 in lanes 2, 3 and 4. Bio-Rad Precision Plus™ Dual Xtra Prestained Protein Standards were run in lane 1. The blot was visualized with the Bio-Rad Chemidoc™ Touch Imaging System.

Western blot

EGF Protein - Western Blot analysis 1 µg recombinant mouse EGF was loaded in lanes 2 and 3, as the primary antibody Goat anti Mouse EGF antibody was used at 0.1 µg/ml and 0.2 µg/ml. HRP conjugated Donkey anti Sheep/Goat IgG antibody was used as the secondary antibody at a dilution of 1/20000 in lanes 2, 3 and 4. Bio-Rad Precision Plus™ Dual Xtra Prestained Protein Standards were run in lane 1. The blot was visualized with the Bio-Rad Chemidoc™ Touch Imaging System.
Western Blot analysis 1 µg recombinant mouse EGF was loaded in lanes 2 and 3, as the primary antibody Goat anti Mouse EGF antibody was used at 0.1 µg/ml and 0.2 µg/ml. HRP conjugated Donkey anti Sheep/Goat IgG antibody was used as the secondary antibody at a dilution of 1/20000 in lanes 2, 3 and 4. Bio-Rad Precision Plus™ Dual Xtra Prestained Protein Standards were run in lane 1. The blot was visualized with the Bio-Rad Chemidoc™ Touch Imaging System.

Western blot

EGF Protein - Western Blot analysis 1 µg recombinant mouse EGF was loaded in lanes 2 and 3, as the primary antibody Goat anti Mouse EGF antibody was used at 0.1 µg/ml and 0.2 µg/ml. HRP conjugated Donkey anti Sheep/Goat IgG antibody was used as the secondary antibody at a dilution of 1/20000 in lanes 2, 3 and 4. Bio-Rad Precision Plus™ Dual Xtra Prestained Protein Standards were run in lane 1. The blot was visualized with the Bio-Rad Chemidoc™ Touch Imaging System.
Western Blot analysis 1 µg recombinant mouse EGF was loaded in lanes 2 and 3, as the primary antibody Goat anti Mouse EGF antibody was used at 0.1 µg/ml and 0.2 µg/ml. HRP conjugated Donkey anti Sheep/Goat IgG antibody was used as the secondary antibody at a dilution of 1/20000 in lanes 2, 3 and 4. Bio-Rad Precision Plus™ Dual Xtra Prestained Protein Standards were run in lane 1. The blot was visualized with the Bio-Rad Chemidoc™ Touch Imaging System.

Western blot

EGF Protein - Western Blot analysis 1 µg recombinant mouse EGF was loaded in lanes 2 and 3, as the primary antibody Goat anti Mouse EGF antibody was used at 0.1 µg/ml and 0.2 µg/ml. HRP conjugated Donkey anti Sheep/Goat IgG antibody was used as the secondary antibody at a dilution of 1/20000 in lanes 2, 3 and 4. Bio-Rad Precision Plus™ Dual Xtra Prestained Protein Standards were run in lane 1. The blot was visualized with the Bio-Rad Chemidoc™ Touch Imaging System.
Western Blot analysis 1 µg recombinant mouse EGF was loaded in lanes 2 and 3, as the primary antibody Goat anti Mouse EGF antibody was used at 0.1 µg/ml and 0.2 µg/ml. HRP conjugated Donkey anti Sheep/Goat IgG antibody was used as the secondary antibody at a dilution of 1/20000 in lanes 2, 3 and 4. Bio-Rad Precision Plus™ Dual Xtra Prestained Protein Standards were run in lane 1. The blot was visualized with the Bio-Rad Chemidoc™ Touch Imaging System.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/21/2024