Target
IL1A / IL-1 Alpha
Synonyms
IL1A | Hematopoietin-1 | IL-1 alpha | IL1 | IL1-ALPHA | IL1 Alpha | Interleukin-1 alpha | IL-1A | IL1F1 | Pro-interleukin-1-alpha | Preinterleukin 1 alpha | Interleukin 1, alpha
Modifications
Carrier-free
Conjugations
Unconjugated
Predicted Molecular Weight
The 156 amino acid recombinant protein has a predicted molecular mass of 17,990 Da. The DTT-reduced and the non-reduced protein migrate at approximately 18kDa by SDS-PAGE. The N-terminal amino acid is Serine.
Purification
Greater than 98% by SDS-PAGE
Usage Summary
Stock solutions can also be prepared at 50-100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA.
Bio-Activity
ED50 is equal to 1- 5 pg/ml, corresponding to a specific activity of 1- 0.2 x 10^9 units/mg, as determined by the dose dependent stimulation of D10S cells proliferation
Endotoxin
Less than 0.01 ng/µg (Less than 0.1 EU/µg) (determined by LAL method).
Presentation
0.22 µm filtered protein solution, pH 7.2, 10 mM NaH2PO4, 150 mM NaCl
Storage
Store unopened at 2°C to 8°C for up to 1 month, at -20°C for up to 6 months, or at -70°C for up to 1 year. Store diluted at -20°C to -70°C. Avoid freeze/thaw cycles. For maximum results, quick spin vial prior to opening.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee