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MCM2

minichromosome maintenance complex component 2

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.

Gene Name: minichromosome maintenance complex component 2
Synonyms: MCM2, BM28, Cell devision cycle-like 1, D3S3194, Cdc19, Cyclin-like 1, KIAA0030, Nuclear protein BM28, CDCL1, MITOTIN
Target Sequences: NM_004526 NP_004517.2 P49736

Publications (3)

1
Role for Plk1 phosphorylation of Hbo1 in regulation of replication licensing. Wu ZQ, Liu X. Proceedings of the National Academy of Sciences of the United States of America. 2008 105:1919-24. [PubMed:18250300] [PMC:PMC2538859]
2
Plk1 phosphorylation of Orc2 promotes DNA replication under conditions of stress. Song B, Liu XS, Davis K, Liu X. Molecular and cellular biology. 2011 31:4844-56. [PubMed:21947279] [PMC:PMC3232917]
3
The opposing transcriptional functions of Sin3a and c-Myc are required to maintain tissue homeostasis. Nascimento EM, Cox CL, MacArthur S, Hussain S, Trotter M, Blanco S, Suraj M, Nichols J, Kbler B, Benitah SA, Hendrich B, Odom DT, Frye M. Nature cell biology. 2011 13:1395-405. (IHC-P; Mouse) [Full Text Article] [PubMed:22101514] [PMC:PMC3242072] Related Antibodies: LS-B391.

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Proteins (5)
Over-Expression Lysate (2)
Recombinant (3)
MCM2 (5)
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GST, N-terminus (1)
His (1)
His-T7 (1)
Myc-DDK (Flag) (2)
Human (4)
293T Cells (1)
E. coli (2)
HEK 293 Cells (1)
Wheat Germ Extract (1)
MCM2 Protein
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E. coli
His
105.8 kD
1 mg/$1,355; 100 µg/$420; 20 µg/$323
MCM2 Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
MCM2 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
101.7 kDa
100 µg/$494
MCM2 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
101.7 kDa
20 µg/$150
MCM2 Protein - Recombinant  Minichromosome Maintenance Deficient 2 By SDS-PAGE
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E. coli
His-T7
26.1 kDa
10 µg/$279; 50 µg/$430; 100 µg/$687; 200 µg/$857; 1 mg/$1,956; 500 µg/$1,499; 5 mg/$2,997; 2 mg/$2,142
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).