Target
Human ADAM17 / TACE
Synonyms
ADAM17 | CD156B | CD156b antigen | CSVP | ADAM 17 | NISBD | TNF-alpha converting enzyme | TNF-alpha convertase | TNF-alpha-converting enzyme | Snake venom-like protease | TACE
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Immunogen
A synthesized peptide derived from human ADAM 17 around the phosphorylation site of Threonine 735.
Specificity
Phospho-ADAM 17 (Thr735) Antibody detects endogenous levels of ADAM 17 only when phosphorylated at Threonine 735.
Applications
- Immunofluorescence (1:100 - 1:500)
- Western blot (1:500 - 1:2000)
- Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee