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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2199-50 | 50 µg (2.2 mg/ml) | $595 |
IHC‑plus™ Polyclonal Sheep anti‑Human EGFR Antibody (Internal, IHC, IF, WB) LS‑B2199
IHC‑plus™ Polyclonal Sheep anti‑Human EGFR Antibody (Internal, IHC, IF, WB) LS‑B2199
Note: This antibody replaces LS-C6555
Antibody:
EGFR Sheep anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP, ELISA
Reactivity:
Human, Mouse, Rat, Chicken
Format:
Unconjugated, Unmodified
Other formats:
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
EGFR Sheep anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP, ELISA
Reactivity:
Human, Mouse, Rat, Chicken
Format:
Unconjugated, Unmodified
Other formats:
Specifications
Description
EGFR antibody LS-B2199 is an unconjugated sheep polyclonal antibody to EGFR (Internal) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, ICC, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human EGFR
Synonyms
EGFR | EGF Receptor | ERBB | ERBB1 | MENA | PIG61 | Proto-oncogene c-ErbB-1 | V-erb-b homolog | EGF-R | HER1
Host
Sheep
Reactivity
Human, Mouse, Rat, Chicken
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant 20aa residues representing a portion of the internal domain of EGFR proximal to the phosphorylation region.
Epitope
Internal
Specificity
Recognizes human Epidermal Growth Factor Receptor.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- ICC
- Immunofluorescence
- Western blot
- Immunoprecipitation
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2199 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2199 was determined to be 5 ug/ml.
Presentation
PBS, pH 7.4, No preservatives added
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About EGFR
Validation
Anti-EGFR antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-EGFR antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ EGFR Antibody (Internal) for IHC, ICC, IF/Immunofluorescence, WB/Western, IP, ELISA LS-B2199 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse, Rat, Chicken
Applications:
IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Species:
Human
Applications:
Flow Cytometry
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/23/2024
Date Requested: 11/23/2024
