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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3470-50 | 50 µl (1 mg/ml) | $460 |
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IHC‑plus™ Monoclonal Mouse anti‑Human KLK3 / PSA Antibody (clone 5A11E2, aa26‑251, IHC, WB) LS‑B3470
IHC‑plus™ Monoclonal Mouse anti‑Human KLK3 / PSA Antibody (clone 5A11E2, aa26‑251, IHC, WB) LS‑B3470
Note: This antibody replaces LS-C41521
Antibody:
KLK3 / PSA Mouse anti-Human Monoclonal (aa26-251) (5A11E2) Antibody
Application:
IHC, IHC-P, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
KLK3 / PSA Mouse anti-Human Monoclonal (aa26-251) (5A11E2) Antibody
Application:
IHC, IHC-P, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
PSA antibody LS-B3470 is an unconjugated mouse monoclonal antibody to human PSA (KLK3) (aa26-251). Validated for ELISA, Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human KLK3 / PSA
Synonyms
KLK3 | APS | Gamma-seminoprotein | Kallikrein-3 | Kallikrein-related peptidase 3 | P-30 antigen | Prostate specific antigen | Prostate-specific antigen | PSA | Semenogelase | KLK2A1 | Seminin
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
5A11E2
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Immunogen
Purified recombinant fragment of KLK3 (aa26-251) expressed in E. coli.
Epitope
aa26-251
Specificity
Purified truncated recombinant KLK3-His(aa26-251) is expressed in E. Coli strain BL21 (DE3)
Applications
- IHC
- IHC - Paraffin (1:200)
- Western blot
- Flow Cytometry (1:200 - 1:400)
- ELISA (1:10000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3470 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3470 was determined to be 1:200.
Presentation
Ascitic fluid, 0.03% sodium azide
Storage
Short term: store at 4°C. Long term: store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About KLK3 / PSA
Validation
Anti-KLK3 / PSA antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-KLK3 / PSA antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
See More About...
LSBio Ratings
IHC-plus™ KLK3 / PSA Antibody (aa26-251, clone 5A11E2) for IHC, WB/Western, Flow, ELISA LS-B3470 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, ICC, Western blot, Flow Cytometry, ELISPOT
Species:
Human
Applications:
IHC, IHC - Paraffin, ELISA, Immunoradiometric assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/20/2024
Date Requested: 11/20/2024
