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Catalog Number Size Price
LS-C97190-400 400 µl $393 
BRAF / B-Raf Antibody - Formalin-fixed and paraffin-embedded human brain tissue reacted with BRAF Antibody (S445), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
BRAF / B-Raf Antibody - Confocal immunofluorescence of B-RAF Antibody (S445) with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
BRAF / B-Raf Antibody - Western blot of BRAF (arrow) using rabbit polyclonal BRAF Antibody (S445) (RB10940). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BRAF gene (Lane 2) (Origene Technologies).
BRAF / B-Raf Antibody - B-RAF Antibody (S445) flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
BRAF / B-Raf Antibody - Formalin-fixed and paraffin-embedded human brain tissue reacted with BRAF Antibody (S445), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
BRAF / B-Raf Antibody - Confocal immunofluorescence of B-RAF Antibody (S445) with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
BRAF / B-Raf Antibody - Western blot of BRAF (arrow) using rabbit polyclonal BRAF Antibody (S445) (RB10940). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BRAF gene (Lane 2) (Origene Technologies).
BRAF / B-Raf Antibody - B-RAF Antibody (S445) flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
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Polyclonal Rabbit anti‑Human BRAF / B‑Raf Antibody (Ser445, IHC, IF, WB) LS‑C97190

Polyclonal Rabbit anti‑Human BRAF / B‑Raf Antibody (Ser445, IHC, IF, WB) LS‑C97190

Antibody:
BRAF / B-Raf Rabbit anti-Human Polyclonal (Ser445) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C97190-400
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
BRAF / B-Raf Rabbit anti-Human Polyclonal (Ser445) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
B-Raf antibody LS-C97190 is an unconjugated rabbit polyclonal antibody to human B-Raf (BRAF) (Ser445). Validated for Flow, IF, IHC and WB.
Target
Human BRAF / B-Raf
Synonyms
BRAF | 94 kDa B-raf protein | B-raf | BRAF1 | B Raf | Proto-oncogene B-Raf | p94 | B-RAF1 | NS7 | ONCOGENE BRAF | RAFB1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Chicken (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
Ser445
Specificity
This B-RAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 424-453 amino acids from human B-RAF.
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BRAF / B-Raf
P15056 NM_004333 NP_004324.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/21/2024