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Antibodies > BRCA1 > LS-C413127
 
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Catalog Number Size Price
LS-C413127-100 100 µl (1 mg/ml) $321 
BRCA1 Antibody - Immunohistochemical analysis of BRCA1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
BRCA1 Antibody - Immunofluorescent analysis of BRCA1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
BRCA1 Antibody - Western blot analysis of BRCA1 expression in K562 (A); HEK293T (B); HT29 (C) whole cell lysates.
BRCA1 Antibody - Immunohistochemical analysis of BRCA1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
BRCA1 Antibody - Immunofluorescent analysis of BRCA1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
BRCA1 Antibody - Western blot analysis of BRCA1 expression in K562 (A); HEK293T (B); HT29 (C) whole cell lysates.
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Polyclonal Rabbit anti‑Human BRCA1 Antibody (C‑Terminus, IHC, IF, WB) LS‑C413127

Polyclonal Rabbit anti‑Human BRCA1 Antibody (C‑Terminus, IHC, IF, WB) LS‑C413127

Submit Review LSBio Guarantee
Antibody:
BRCA1 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$321
LS-C413127-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
BRCA1 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
BRCA1 antibody LS-C413127 is an unconjugated rabbit polyclonal antibody to human BRCA1 (C-Terminus). Validated for ICC, IF, IHC and WB.
Target
Human BRCA1
Synonyms
BRCA1 | BROVCA1 | BRCC1 | Breast cancer 1, early onset | PPP1R53 | PSCP | PNCA4 | RING finger protein 53 | RNF53 | BRCAI | Breast cancer 1 early onset | IRIS
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human BRCA1.
Epitope
C-Terminus
Specificity
Recognizes endogenous levels of BRCA1 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BRCA1
P38398 NM_007294 NP_009225.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/4/2024
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