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Catalog Number	Size	Price
LS-C413127-100	100 µl (1 mg/ml)	$321

BRCA1 Antibody - Immunohistochemical analysis of BRCA1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

BRCA1 Antibody - Immunofluorescent analysis of BRCA1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

BRCA1 Antibody - Western blot analysis of BRCA1 expression in K562 (A); HEK293T (B); HT29 (C) whole cell lysates.

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Polyclonal Rabbit anti‑Human BRCA1 Antibody (C‑Terminus, IHC, IF, WB) LS‑C413127

Name: Anti-BRCA1 Antibody | Rabbit anti-Human Polyclonal ICC,IF,IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C413127-100
Price: 321.0000 USD
Availability: InStock

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Antibody:

BRCA1 Rabbit anti-Human Polyclonal (C-Terminus) Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human

Format:

Unconjugated, Unmodified

$321

LS-C413127-100

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

BRCA1 Rabbit anti-Human Polyclonal (C-Terminus) Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

BRCA1 antibody LS-C413127 is an unconjugated rabbit polyclonal antibody to human BRCA1 (C-Terminus). Validated for ICC, IF, IHC and WB.

Target

Human BRCA1

Synonyms

See All BRCA1 Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunoaffinity purified

Modifications

Unmodified

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human BRCA1.

Epitope

C-Terminus

Specificity

Recognizes endogenous levels of BRCA1 protein.

Applications

IHC
IHC - Paraffin (1:100 - 1:200)
ICC (1:100 - 1:500)
Immunofluorescence (1:100 - 1:500)
Western blot (1:500 - 1:1000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol

Storage

Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About BRCA1

Uniprot: P38398 NCBI: NM_007294 NP_009225.1