Target
Human CASP3 / Caspase 3
Synonyms
CASP3 | Apopain | CASP-3 | Caspase-3 | CPP32B | CPP32 | Cysteine protease CPP32 | SCA-1 | SREBP cleavage activity 1 | Yama | Procaspase3 | Caspase 3 | CPP-32 | PARP cleavage protease | Protein Yama
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
Synthetic peptide corresponding residues adjacent to Asp175 in N-Terminus of human caspase-3 (KLH coupled).
Specificity
Recognizes cleaved human Caspase 3. Detects endogenous levels of the large fragment (17/19kD) of activated caspase-3 resulting from cleavage adjacent to Asp175. Does not recognize full length caspase-3 or other cleaved caspases. Species cross-reactivity: mouse, bovine, and rat.
Applications
- IHC
- IHC - Frozen (1:200)
- ICC
- Immunofluorescence
- Western blot (1:1000)
- Flow Cytometry (1:200)
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Usage
Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunohistochemistry, Immunocytochemistry and Flow Cytometry. Recommend Dilution: Western Blot: 1:1000. Immunohistochemistry (Frozen): 1:200. Immunocytochemistry (IF):1:200. Flow Cytometry: 1:200.
Presentation
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/mL BSA, 50% Glycerol
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee