Mouse CD3E

Hamster

Mouse (tested or 100% immunogen sequence identity)

IgG Monoclonal

145-2C11

Allophycocyanin. Also available Unconjugated or conjugated with RPE, Biotin, FITC.

Protein G purified

Unmodified

Mouse BM10-37 T cell membrane prep.

Antibody 145-2C11binds to an extracellular epitope of the 25 kd CD3e molecule present on murine T cells.

• Western blot
• Immunoprecipitation
• Flow Cytometry
• ELISA
• Applications tested for the base form of this product only

Reagent was tested for binding to ACK lysed murine splenocytes in FACS. Five x 105 splenocytes per tube were washed and pre incubated with 20 ul of 300 ug/ml Mouse IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 ul of anti-Mouse CD3/APC at a 1:50 dilution (5 ug/ml). They were then washed three times, fixed and analyzed by FACS. A 44.4% sub population of the cells stained positive with a mean shift of 1.35 log10 fluorescent units when compared to background. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-mouse CD3 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 500 mM KCl, 0.04% Sodium Azide, 15% Glycerol, 0.2% BSA

Store at 4°C. Do not freeze. Protect from light.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

NCBI: NM_000733 NP_000724.1