Mouse CD3E

Hamster

Mouse (tested or 100% immunogen sequence identity)

IgG Monoclonal

145-2C11

Unconjugated. Also available conjugated with RPE, Biotin, APC, FITC.

Protein G purified

Unmodified

Mouse BM10-37 T cell membrane prep.

Antibody 145-2C11binds to an extracellular epitope of the 25 kd CD3e molecule present on murine T cells.

• Western blot
• Immunoprecipitation
• Flow Cytometry
• ELISA

Reagent was tested for binding to ACK lysed murine splenocytes in FACS. Five x 105 splenocytes per tube were washed and pre incubated with 20ul of 300ug/ml murine IgG (to reduce non specific binding) after which they were incubated 45 minutes on ice with 80 ul of anti-CD3 antibody diluted to 10 ug/ml. Cells were washed twice and incubated with 2o reagent Goat anti-Hampster IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. A 33% sub population of the cells stained positive with a mean shift of 1.08 log10 fluorescent units when compared to background.

50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.5 mg/ml Gentamicin

Store at 4°C, avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

NCBI: NM_000733 NP_000724.1