Mouse CD3E

Hamster

Mouse (tested or 100% immunogen sequence identity)

IgG Monoclonal

145-2C11

Biotin. Also available Unconjugated or conjugated with RPE, APC, FITC.

Protein G purified

Unmodified

Mouse BM10-37 T cell membrane prep.

Antibody 145-2C11binds to an extracellular epitope of the 25 kd CD3e molecule present on murine T cells.

• Western blot
• Immunoprecipitation
• Flow Cytometry
• ELISA
• Applications tested for the base form of this product only

Reagent was tested for binding to ACK lysed murine splenocytes in FACS. Five x 105 splenocytes per tube were washed and pre incubated 5 minutes with 20 ^l of 250 ug/ml Mouse IgG (to reduce non specific binding) after which they were incubated 45 minutes on ice with 80 ul of anti-mouse CD3/Biotin at concentration of 10 ^g/ml. They were then washed twice and incubated with 2o reagent Streptavidin/R-PE after which they were washed twice, fixed and analyzed by FACS. A 35% sub population of the cells stained positive with a mean shift of 1.34 log10 fluorescent units when compared to background. Binding was blocked when cells were pre incubated with 20^l of 0.5mg/ml unlabeled anti-CD3 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.04% Sodium Azide, 5% Glycerol, 0.2% BSA

Store at 4°C, avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

NCBI: NM_000733 NP_000724.1