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Catalog Number Size Price
LS-C313534-10 10 µg $318 
LS-C313534-100 100 µg (0.5 mg/ml) $470 
CMA1 / Mast Cell Chymase Antibody - Immunohistochemistry - Anti-CMA1/Chymase Picoband Antibody
CMA1 / Mast Cell Chymase Antibody - Western blot analysis of CMA1 using anti-CMA1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CMA1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CMA1 at approximately 34KD. The expected band size for CMA1 is at 28KD.
CMA1 / Mast Cell Chymase Antibody - Western blot - Anti-CMA1/Chymase Picoband Antibody
CMA1 / Mast Cell Chymase Antibody - Immunohistochemistry - Anti-CMA1/Chymase Picoband Antibody
CMA1 / Mast Cell Chymase Antibody - Western blot analysis of CMA1 using anti-CMA1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CMA1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CMA1 at approximately 34KD. The expected band size for CMA1 is at 28KD.
CMA1 / Mast Cell Chymase Antibody - Western blot - Anti-CMA1/Chymase Picoband Antibody
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Polyclonal Rabbit anti‑Human CMA1 / Mast Cell Chymase Antibody (aa22‑247, IHC, WB) LS‑C313534

Polyclonal Rabbit anti‑Human CMA1 / Mast Cell Chymase Antibody (aa22‑247, IHC, WB) LS‑C313534

Antibody:
CMA1 / Mast Cell Chymase Rabbit anti-Human Polyclonal (aa22-247) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C313534-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CMA1 / Mast Cell Chymase Rabbit anti-Human Polyclonal (aa22-247) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Mast Cell Chymase antibody LS-C313534 is an unconjugated rabbit polyclonal antibody to human Mast Cell Chymase (CMA1) (aa22-247). Validated for IHC and WB.
Target
Human CMA1 / Mast Cell Chymase
Synonyms
CMA1 | Chymase | Chymase, heart | Chymase, mast cell | CYH | Mast cell chymase | Mcpt5 | MCT1 | Mast cell protease 5 | Mast cell protease I | Heart chymase | Alpha-chymase | Chymase 1 | Chymase 1, mast cell | CYM
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human CMA1 recombinant protein (Position: I22-N247). Human CMA1 shares 75% and 74% amino acid (aa) sequences identity with mouse and rat CMA1, respectively.
Epitope
aa22-247
Specificity
Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions. .
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CMA1 / Mast Cell Chymase
P23946 NM_001836 NP_001827.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/1/2024