Human GFAP

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Recombinant Monoclonal

rASTRO/789

Unconjugated

Protein A/G purified

Azide-free, Carrier-free. Also available Unmodified.

Recombinant full-length human GFAP protein. Antigen Molecular Weight: ~50 kDa

This MAb recognizes a protein of ~50 kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

• IHC - Paraffin (0.25 - 0.5 µg/ml)
• Western blot (0.5 - 1 µg/ml)
• Flow Cytometry (1 - 2 µg/10E6 cells)

Western Blotting (0.5-1 ug/ml) - Immunohistology (Formalin-fixed - 0.25-0.5 ug/ml for 30 minutes at RT - Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.) Optimal dilution for a specific application should be determined.

10 mM PBS

Store at -20°C to -80°C for up to 2 years.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P14136 NCBI: NM_002055 NP_002046.1