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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2192-50 | 50 µg (0.5 mg/ml) | $485 |
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PathPlus™ Polyclonal Goat anti‑Human GFAP Antibody (aa417‑430, IHC, WB) LS‑B2192
PathPlus™ Polyclonal Goat anti‑Human GFAP Antibody (aa417‑430, IHC, WB) LS‑B2192
Note: This antibody replaces LS-C34686, LS-C66495
Antibody:
GFAP Goat anti-Human Polyclonal (aa417-430) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Rat, Bat, Bovine, Horse, Rabbit
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
GFAP Goat anti-Human Polyclonal (aa417-430) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Rat, Bat, Bovine, Horse, Rabbit
Format:
Unconjugated, Unmodified
Specifications
Description
GFAP (glial fibrillary acidic protein) is an intermediate filament protein found in the central nervous system. Functionally, GFAP helps maintain cell shape and astrocyte mechanical strength, as well as participate in cell communication and blood brain barrier function. In immunohistochemistry, it is positive in astrocytes, ganglion cells and some ependymal cells of the central nervous system. In cancer, it is positive in ependymomas and in astrocytic tumors such as astrocytic gliomas, astrocytomas and glioblastomas.
References: Brain Research. 2007 July. 1158: 103–15. doi:10.1016/j.brainres.2007.04.070, PMID: 17555726; Neurology India. 2013. 61 (4): 383–8, PMID: 24005729; The Journal of Cell Biology. 1991 Mar. 112 (6): 1205–13. doi:10.1083/jcb.112.6.1205, PMID: 1999469; Neurosci Lett. 1996 May 3;209(1):29-32, PMID: 8734902.
Host
Goat
Reactivity
Human, Monkey, Rat, Bat, Bovine, Horse, Rabbit
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-DGEVIKESKQEHKD, from the C-Terminus of protein sequence according to NP_002046.1.
Epitope
aa417-430
Specificity
Human GFAP.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (0.01 - 0.1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2192 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2192 was determined to be 5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About GFAP
Validation
Anti-GFAP antibody IHC of human brain, cortex, astrocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-GFAP antibody IHC of human brain, cortex, astrocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
PathPlus™ GFAP Antibody (aa417-430) for IHC, WB/Western LS-B2192 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
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Featured Products
Species:
Pig, Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
Species:
Pig, Human, Feline, Porcine
Applications:
IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Species:
Mouse, Human, Rat
Applications:
IHC, Immunofluorescence, Western blot
Species:
Human
Applications:
IHC - Paraffin, Flow Cytometry
Species:
Human
Applications:
IHC - Paraffin, Western blot, Flow Cytometry
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
