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Catalog Number Size Price
LS-C748687-20 20 µl $275 
LS-C748687-100 100 µl $389 
IFIH1 / MDA5 Antibody - Immunofluorescence analysis of A549 cells using IFIH1 antibody. Blue: DAPI for nuclear staining.
IFIH1 / MDA5 Antibody - Western blot analysis of extracts of THP-1 cells, using IFIH1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
IFIH1 / MDA5 Antibody - Immunofluorescence analysis of A549 cells using IFIH1 antibody. Blue: DAPI for nuclear staining.
IFIH1 / MDA5 Antibody - Western blot analysis of extracts of THP-1 cells, using IFIH1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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Polyclonal Rabbit anti‑Human IFIH1 / MDA5 Antibody (IF, WB) LS‑C748687

Polyclonal Rabbit anti‑Human IFIH1 / MDA5 Antibody (IF, WB) LS‑C748687

Antibody:
IFIH1 / MDA5 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C748687-20
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
IFIH1 / MDA5 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
MDA5 antibody LS-C748687 is an unconjugated rabbit polyclonal antibody to MDA5 (IFIH1) from human. It is reactive with human and mouse. Validated for IF and WB.
Target
Human IFIH1 / MDA5
Synonyms
IFIH1 | Helicase with 2 CARD domains | MDA-5 | MDA5 | Helicard | IDDM19 | RLR-2 | RH116 | CADM-140 autoantigen | Hlcd | RIG-I-like receptor 2
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human IFIH1 (NP_071451.2). MSNGYSTDENFRYLISCFRARVKMYIQVEPVLDYLTFLPAEVKEQIQRTVATSGNMQAVELLLSTLEKGVWHLGWTREFVEALRRTGSPLAARYMNPELTDLPSPSFENAHDEYLQLLNLLQPTLVDKLLVRDVLDKCMEEELLTIEDRNRIAAAENNGNESGVRELLKRIVQKENWFSAFLNVLRQTGNNELVQELTGSDCSES
Applications
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Usage
The predicted MW is 25kDa/116kDa, while the observed MW by Western blot was 117kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IFIH1 / MDA5
Q9BYX4 NM_022168 NP_071451.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/25/2024