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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2777-50 | 50 µl (2.4 mg/ml) | $485 |
IHC‑plus™ Monoclonal Mouse anti‑Human NES / Nestin Antibody (aa1464‑1614, IHC, IF, WB) LS‑B2777
IHC‑plus™ Monoclonal Mouse anti‑Human NES / Nestin Antibody (aa1464‑1614, IHC, IF, WB) LS‑B2777
Note: This antibody replaces LS-C73314
Antibody:
NES / Nestin Mouse anti-Human Monoclonal (aa1464-1614) Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
NES / Nestin Mouse anti-Human Monoclonal (aa1464-1614) Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
Nestin antibody LS-B2777 is an unconjugated mouse monoclonal antibody to human Nestin (NES) (aa1464-1614). Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Non-Reactivity
Rodent
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
A fragment of the human nestin protein corresponding to residues 1464-1614.
Epitope
aa1464-1614
Specificity
This antibody is specific for human nestin. Species cross-reactivity: This antibody recognizes human nestin protein. It does not cross-react with rodent protein.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- IHC - Frozen
- ICC
- Immunofluorescence
- Western blot (1:1000 - 1:5000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2777 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2777 was determined to be 5 ug/ml. Western Blot: 1:1000-1:5000. Doublet band seen ~220-240kD.
Presentation
PBS, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About NES / Nestin
Validation
Anti-Nestin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Nestin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ NES / Nestin Antibody (aa1464-1614) for IHC, ICC, IF/Immunofluorescence, WB/Western LS-B2777 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Featured Products
Species:
Rat, Mouse
Applications:
IHC, IHC - Frozen, ICC, Western blot
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Rat
Applications:
IHC, IHC - Frozen, Immunofluorescence, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/24/2024
Date Requested: 11/24/2024
