Human NF-L / NEFL

Chicken

Human (tested or 100% immunogen sequence identity)

IgY Polyclonal

Unconjugated

Purified

Unmodified

To raise this antibody bovine intermediate filaments were prepared from spinal cords by the method of Delacourte et al. and the cytoskeletal material was dissolved in 6M urea. Individual neurofilament subunits were purified by ion exchange chromatography on DEAE cellulose followed by preparative gel electrophoresis. To ensure greater specificity for NF-L, animals were boosted with recombinant mouse NF-L purified from bacteria.

Human NF-L / NEFL

• IHC
• IHC - Paraffin (1:500)
• ICC (1:5000)
• Immunofluorescence (1:5000)
• Western blot (1:10000)

The IgY solution can be used at dilutions of at least 1:5000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:10000 or lower.

10 mM Sodium Azide

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P07196 NCBI: NM_006158 NP_006149.2