Human p16INK4a / CDKN2A

Rabbit

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Delipidated and defibrinated

Unmodified

Human p16 protein (missing the first 7 amino acids)

This product was prepared from monospecific antiserum by delipidation and defibrination. Antiserum will specifically react with a p16 protein from human tissue. Cross reactivity may occur with p15 when immunoprecipitating in vitro translated product but not in immunoblots. Potentially cross reacts with p15.5.

• IHC
• IHC - Paraffin (1:500)
• Western blot (1:500 - 1:5000)
• Immunoprecipitation (1:100)
• ELISA (1:5000 - 1:20000)

Immunohistochemistry: LS-B647 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B647 was determined to be 1:500.

Antiserum, 0.01% Sodium Azide

Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.